刘敏,刘爱萍, 韩海斌,甘霖,陈国泽,黄海广,2021,茶足柄瘤蚜茧蜂碳水化合物代谢相关的滞育关联基因差异表达分析[J].环境昆虫学报,(1):147-157
茶足柄瘤蚜茧蜂碳水化合物代谢相关的滞育关联基因差异表达分析
Differential expression of genes associated with carbohydrate metabolism in diapausing Lysiphlebus testaceipes Cresson
  
DOI:
中文关键词:  茶足柄瘤蚜茧蜂  滞育  差异表达基因  碳水化合物代谢
英文关键词:Lysiphlebus testaceipes Cresson  diapause  differentially expressed genes  carbohydrate metabolism
基金项目:国家重点研发项目(2017YFD02010000);国家重点研发项目政府间国际科技创新合作重点专项(2017YFE0104900)
作者单位
刘敏,刘爱萍, 韩海斌,甘霖,陈国泽,黄海广 1. 中国农业科学院草原研究所呼和浩特 0100102. 阿拉善科技信息研究所阿拉善左旗7503993. 阿拉善右旗曼德拉苏木农牧业综合站阿拉善右旗7503004. 内蒙古自治区林业科学研究院呼和浩特 010010 
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中文摘要:
      为明确糖代谢相关途径在茶足柄瘤蚜茧蜂Lysiphlebus testaceipes蛹滞育过程中的作用,揭示滞育调控的分子机制。本试验利用转录组测序技术,对滞育组与非滞育组的茶足柄瘤蚜茧蜂蛹进行转录组测序,并结合生物信息学方法对糖代谢相关途径中的差异表达基因进行了筛选与分析。GO注释到的与碳水化合物代谢条目相关的差异基因共1 050个,KEGG注释到的与碳水化合物代谢相关的差异表达基因共149个,糖酵解/糖异生、淀粉与蔗糖代谢及柠檬酸循环三条途径的差异表达基因分别为18、10和18个。这些在滞育过程中与碳水化合物代谢相关的差异基因呈现不同程度的上调或下调表达,发现PFK,PGK,ALDO,GAPDH,PGAM,PEPCK,GYS,TreS,TreH,MDH,IDH等基因与茶足柄瘤蚜茧蜂滞育密切相关,共同影响茶足柄瘤蚜茧蜂的滞育。碳水化合物代谢相关途径可能对茶足柄瘤蚜茧蜂的滞育起着非常重要的作用,糖类物质的合成与分解为昆虫在滞育过程中提供能量。
英文摘要:
      To clarify the role of carbohydrate metabolism in Lysiphlebus testaceipes Cresson can help us to reveal the molecular mechanism in diapause process, we took advantage of transcriptome sequencing technology to sequence the pupae of the diapause group and the non-diapause group, and combined with the Bioinformatics Method to screen and analyze the differentially expressed genes (DEGs) in carbohydrate metabolism. 1 050 DEGs related to carbohydrate metabolism items annotated in GO database. KEGG database annotated a total of 149 DEGs related to carbohydrate metabolism, focusing on glycolysis/ gluconeogenesis, starch and sucrose metabolism, citric acid cycle. DEGs were 18, 10 and 18, respectively. These DEGs associated with carbohydrate metabolism during diapause exhibited different levels of up-regulation or down-regulation expression, and it was found that PFK, PGK, ALDO, GAPDH, PGAM, PEPCK, GYS, TreS, TreH, MDH, IDH genes were closely related to the diapause of L. testaceipes, which affected the diapause of the insect. Carbohydrate metabolism played an important role in the diapause of L. testaceipes, and the synthesis and decomposition of the carbohydrate substances provided energy for the insect in diapause process, and also took part in the aspects of antifreeze, protein protection, reducing osmotic pressure or supercooling point.
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