| 刘敏，韩海斌，刘爱萍，高书晶，徐林波，黄海广,2020,茶足柄瘤蚜茧蜂蛹滞育过程中胰岛素信号通路及其相关途径的初探[J].环境昆虫学报,(6):1439-1445 |
| 茶足柄瘤蚜茧蜂蛹滞育过程中胰岛素信号通路及其相关途径的初探 |
| A preliminary study on the insulin signaling pathway and its related pathways in diapause of Lysiphlebus testaceipes Cresson |
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| DOI： |
| 中文关键词: 茶足柄瘤蚜茧蜂 滞育 差异表达基因 胰岛素信号通路 |
| 英文关键词:Lysiphlebus testaceipes Cresson diapause differentially expressed genes Insulin signaling pathway |
| 基金项目:国家重点研发项目（2017YFD02010000）；国家重点研发项目政府间国际科技创新合作重点专项（2017YFE0104900） |
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| 中文摘要: |
| 本实验通过探索胰岛素信号通路及其相关途径对茶足柄瘤蚜茧蜂蛹滞育的影响，从而方便寻找胰岛素替代物，为害虫防治提供新思路。利用RNA-Seq，对滞育组与非滞育组的茶足柄瘤蚜茧蜂进行转录组测序，结合生物信息学方法对转录组中胰岛素信号通路及其相关途径的差异表达基因进行了分析。与胰岛素信号通路相关差异表达基因共31个，重点分析的PI3K-Akt, FoxO, MAPK三条途径，差异表达基因分别为55, 21和28个。这些滞育关联基因呈现不同程度的上调或下调表达，发现Sos, FASN, TSC1, PRKAB等基因与茶足柄瘤蚜茧蜂滞育密切相关，共同影响茶足柄瘤蚜茧蜂的滞育。胰岛素信号通路及其相关途径对茶足柄瘤蚜茧蜂的滞育起着非常重要的作用，主要体现在影响虫体能量代谢、脂质积累、细胞增殖等方面。 |
| 英文摘要: |
| To clarify the role of insulin signaling pathway and its related pathways in diapause of Lysiphlebus testaceipes Cresson, so as to search for insulin substitutes and provide new ideas for pest control. In this experiment, we used RNA-seq technology to sequence the transcriptome of L. testaceipes from diapause and non-diapause groups, and the differentially expressed genes of insulin signaling pathway and its related pathways were analyzed by bioinformatics. There were 31 differentially expressed genes related to insulin signaling pathway, among which, PI3K Akt, FOXO and MAPK were the three major pathways, with 55, 21 and 28 differentially expressed genes, respectively. The genes of Sos, FASN, TSC1 and PRKAB were closely related to diapause of L. testaceipes, which jointly affected the diapause. Insulin signaling pathway and its related pathways play a very important role in diapause of L. testaceipes, which mainly affected energy metabolism, lipid accumulation, cell proliferation. |
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