鲁智慧,陈亚平,周叆春,和淑琪,李浩,包禹优,桂富荣,2020,不同寄主植物继代饲养后草地贪夜蛾幼虫生理酶活性差异[J].环境昆虫学报,(6):1361-1368
不同寄主植物继代饲养后草地贪夜蛾幼虫生理酶活性差异
Effects of multi-generation feeding with different host plants on activities of enzyme in Spodoptera frugiperda larvae
  
DOI:
中文关键词:  草地贪夜蛾  消化酶  保护酶  解毒酶  次生代谢物
英文关键词:Spodoptera frugiperda  digestive enzymes  protective enzymes  detoxification enzymes  secondary metabolites
基金项目:云南省科技重大专项云南省绿色食品国际合作研究中心(YEFICRC)项目(2019ZG00910); 国家重点研发计划(2019YFD0300101)
作者单位
鲁智慧,陈亚平,周叆春,和淑琪,李浩,包禹优,桂富荣 1. 云南农业大学植物保护学院 省部共建云南生物资源保护与利用国家重点实验室昆明 6502012. 云南省植保植检站昆明 6500343. 云南省高原特色农业产业研究院昆明 650201 
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中文摘要:
      为了明确继代饲喂不同寄主植物对草地贪夜蛾幼虫体内生理酶活性的影响,在室内分别用3种寄主植物叶片饲养草地贪夜蛾多代,测定其F3代5龄幼虫体内保护酶(SOD,POD,CAT)、消化酶(脂肪酶、胃蛋白酶和α-淀粉酶)和解毒酶(GSTs,CarEs,CYP450s)活性差异,并分析不同寄主植物次生代谢物含量与幼虫体内酶活及其生长发育的相关性。结果表明,取食马铃薯叶片的F3代幼虫总发育历期最长,取食小麦的最短,两者间差异显著;小麦叶片中的单宁、总酚、类黄酮和生物碱含量均显著低于玉米和马铃薯;草地贪夜蛾幼虫体内保护酶活性均为取食小麦叶片的最低,取食马铃薯叶片的最高,其中取食马铃薯叶片的草地贪夜蛾体内POD和SOD酶活性达取食小麦的1.34和1.26倍;以3种寄主为食的F3代幼虫脂肪酶和α-淀粉酶活性变化不大,但持续饲喂马铃薯叶片3个世代可导致草地贪夜蛾幼虫体内胃蛋白酶活性显著升高,达10.502 U/mg. prot,为持续饲喂玉米叶片幼虫(1.508 U/mg. prot)的6.96倍以上。连续饲喂3种寄主叶片后的幼虫GSTs和CarEs活性无显著性差异,但取食玉米叶片的幼虫体内细胞色素P450s(CYP450s)活性显著高于取食小麦和马铃薯的幼虫。可见,连续多代取食次生物质含量不同的寄主植物会使草地贪夜蛾体内部分酶活性发生变化,并对幼虫的生长发育产生影响。
英文摘要:
      To fully understand the impact of multi-generation feeding with different host plants on the activities of enzymes in fall armyworm (FAW), Spodoptera frugiperda larvae, referred to as the leaves of three host plants (maize, wheat and otato) were used as the food for FAW larvae from the first generation to the third generations of 5th instar, and the activities of protective enzymes (superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)), digestive enzymes (lipase, pepsin and α-amylase) and detoxification enzymes (glutathione S-transferase (GSTs), carboxylesterase (CarEs) and cytochrome P450s (CYP450s) were determined in the 5th instar of S. frugiperda, the analysis of the correlations among the development, the enzymes activities in FAW larvae and the concentrations of secondary metabolites in host plants were also conducted. The results showed that the larval development duration was longest when fed on potato leaves and shortest fed on wheat leaves, and the difference was significant. The concentrations of total phenols, tannins, flavonoids and alkaloid in the maize and potato leaves were significantly higher than those in wheat. The activities of CAT, POD and SOD in larvae fed on potato leaves were highest, while those in the larvae fed on wheat leaves were lowest. Besides, the POD and SOD activities in larvae fed on potato leaves were 1.34 and 1.26 times higher than those in larvae fed on wheat leaves, while the activities of pepsin in larvae fed on potato leaves (10.502 U/mg. prot) were more than 6.96 times of those in larvae fed on maize leaves (1.508 U/mg. prot), and there was no significant difference in the activities of lipase and α-amylase between FAW that fed on three host plant leaves. Moreover, the activities of CYP450s in S. frugiperda fed on maize leaves were significantly higher than that fed on wheat and potato leaves, however, the activities of GSTs and CarEs larvae those fed on different host plant leaves had no significant difference. In summary, multi-generation feeding with different host plants may has different concentrations of secondary metabolites, and the activities of enzymes in FAW had changed and the development duration of larvae were different.
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