《环境昆虫学报》

作者	单位
叶佳琴，郅军锐，曾广，张涛，黄宸，周丹	贵州大学昆虫研究所，贵州省山地农业病虫害重点实验室，贵阳 550025

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中文摘要:

为阐明抗生素对西花蓟马的作用，选用氨苄青霉素(AMP)、氯霉素(CAP)和硫酸链霉素(SM)等3种抗生素分别采用薄膜饲喂法和叶片浸渍法处理西花蓟马，研究3种抗生素对西花蓟马死亡率及对肠道可培养细菌的影响。结果表明抗生素对西花蓟马的死亡率有明显的影响，随着浓度的增加和处理时间的延长死亡率升高，在薄膜饲喂法和叶片浸渍法处理下，西花蓟马均是在3种抗生素浓度为50.00 mg/mL处理72 h时死亡率最大，薄膜饲喂法对西花蓟马死亡率的影响高于叶片浸渍法。在2种饲喂方式下，25.00 mg/mL氨苄青霉素和50.00 mg/mL硫酸链霉素处理72 h后对西花蓟马肠道内可培养细菌的去除效果最好；浓度为50.00 mg/mL氯霉素在薄膜饲喂法中处理24 h后对西花蓟马肠道细菌去除效果最好，但叶片浸渍法没有明显的作用。结果说明，西花蓟马死亡率及肠道细菌的去除效果与抗生素种类、浓度和处理时间相关，也与处理方法有关。

英文摘要:

To explore the effect of different antibiotics on Frankliniella occidentalis , Ampicillin (AMP), chloramphenicol (CAP) and streptomycin sulphate (SM) were used to treat F. occidentalis by membrane feeding and leaf dipping methods respectively. The influences of three kinds of antibiotics on the mortality and the removal effects on the culturable gut bacteria in F.occidentalis were determined. The results showed that the antibiotics had significant effect on the mortality of F.occidentali, which increased with the increase of concentration and extension of time. The mortality was biggest under the concentration of 50.00 mg/mL of all 3 antibiotics at 72 h at both methods. The membrane feeding method had stronger effect than leaf dipping method 25.00 mg/mL ampicillin and 50.00 mg/mL streptomycin sulfate had best remove effects on culturable bacteria in the gut of F.occidentalis under the two methods. The concentration of chloramphenicol with 50.00 mg/mL at 24 h had biggest remove effect at the membrane feeding method, while it hadn't the effect at leaf dipping method. The results indicated that the effect of antibiotics on the mortality of F.occidentalis and the removal effect were positively correlated with antibiotic type, concentration and treatment time, it also had the relative with treatment method.

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