| 李新宇，马卫华，李立新，申晋山，杜亚丽，龙登隆，张 琪，刘晋佳，王春梅，兰 俊，冯宇佳，姜玉锁,2020,中华蜜蜂PLA2基因的生物信息学分析及响应高温胁迫的表达模式[J].环境昆虫学报,(5):1060-1067 |
| 中华蜜蜂PLA2基因的生物信息学分析及响应高温胁迫的表达模式 |
| Bioinformatics analysis of PLA2 gene and expression Characteristcs in high temperature stress in Apis cerana cerana |
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| DOI： |
| 中文关键词: 中华蜜蜂 PLA2 高温胁迫 表达模式 |
| 英文关键词:Apis cerana cerana PLA2 high-temperature stress expression pattern |
| 基金项目:现代农业产业技术（蜜蜂）体系建设（CARS-44-KXJ23）；山西省农业科学院农业科技创新基金（YCX2018203）；山西省高等教育振兴计划暨1331工程 |
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| 中文摘要: |
| 磷脂酶A2 (PLA2)对细胞膜流动性、膜蛋白活性等细胞生理功能有重要调节作用。高温等环境压力对细胞的生理代谢有较大的影响，其在生物体抗逆过程中的调控作用一直备受关注。本研究以中华蜜蜂Hymenoptera: Apidae: Apis cerana cerana PLA2基因序列为基础，对其蛋白结构进行预测，分析该基因在不同温度和高温不同胁迫时间下的表达差异，以揭示该基因在中华蜜蜂抗高温过程中的生理功能。结果显示，中华蜜蜂PLA2基因包含745 bp的开放阅读框，编码169个氨基酸，蛋白分子量为19.3 kDa，无跨膜结构，不属于膜蛋白。氨基酸同源序列比对结果显示，中华蜜蜂PLA2序列与蜜蜂科昆虫的相似性最高，与其他膜翅目昆虫的相似性存在差异。qRT-PCR结果显示，PLA2在35℃、40℃和45℃处理下的表达量较高，较高的温度能诱导该基因表达的增加。同时，PLA2的表达受高温胁迫时间的影响，较长时间的高温胁迫导致PLA2基因的表达增加。本研究结果表明PLA2在中华蜜蜂应对高温胁迫时发挥重要生理功能。 |
| 英文摘要: |
| PLA2 plays an important role in regulating the cell physiological functions such as cell membrane fluidity and membrane protein activity. Environmental stress such as high temperature has a great influence on the physiological metabolism of cells, and its regulation in the process of anti-reverse organisms has been receiving much attention. According to the AcerPLA2 gene sequence of Hymenoptera: Apidae: Apis cerana cerana, the protein structure was predicted, and its expression difference at different temperature and different high temperature stress time was analyzed to reveal the physiology of this gene in the process of Apis cerana cerana against high temperature. The AcerPLA2 gene of Apis cerana cerana contained 745 bp open reading frame encoding 169 amino acids with a molecular weight of 19.3 kDa, no transmembrane structure, and no membrane protein. The amino acid homologous sequence alignment showed that the AcerPLA2 sequence had the highest similarity with the honeybee insects, and the similarity with other Hymenoptera insects. The results of qRT-PCR showed that the expression of PLA2 was higher at 35℃, 40℃ and 45℃, and higher temperature could induce the increase of the expression of the gene. At the same time, the expression of PLA2 was affected by the time of high temperature stress, and the long-term high temperature stress caused the expression of PLA2 gene to increase.The results showed that AcerPLA2 played important physiological functions in Apis cerana cerana in response to high temperature stress. |
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