《环境昆虫学报》

作者	单位
胡景华，赵方媛，张丽珍，江武军，廖春华，颜伟玉	1. 江西农业大学蜜蜂研究所，南昌 330045；2.江西省养蜂研究所，南昌 330052

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中文摘要:

为研究育王过程中添加蜜蜂幼虫信息素成分甲基硬脂酸酯和E-β-罗勒烯对中华蜜蜂育王质量的影响，本试验结合免移虫育王技术，在幼虫60~64 h时向王台内注入1 μL配置好的信息素（浓度梯度分别为0%、0.1%、1.0%、10.0%），待蜂王出房后测定蜂王个体指标及蜂王卵巢 Vg、hex70b 和hex110基因的表达水平。与空白对照相比，添加10.0%浓度的 E-β-罗勒烯组蜂王的初生重和单侧卵巢管数显著增加，同时Vg、hex70b 和 hex110 基因表达水平也显著上升；添加1.0%的 E-β-罗勒烯蜂王单侧卵巢管数量显著增加，Vg 和 hex110 基因的表达量也显著上升；添加甲基硬脂酸酯对培育蜂王的初生重、胸部指标和卵巢相关基因表达均无显著影响，但1.0%和10.0%的甲基硬脂酸酯使蜂王卵巢管数量显著减少。结果表明在中蜂育王过程中添加 E-β-罗勒烯可以在一定程度上提高蜂王的质量

英文摘要:

In order to explore the effects of the components of brood pheromone, methyl stearate (MS) andE-beta-ocimene (eβ), on the reared queen quality of Apis cerana cerana, 1 μL of compound with the concentration gradients of 0%, 0.1%, 1.0% and 10.0% was added into queen cells respectively using a micro sampling syringe injector when queen larvae was 60~64 h old. Newly emerged queens were sampled and analyzed for the index of queens and the expression level of Vg, hex70b and hex110 genes in queen ovaries. Compares with the blank control group, 10.0% treatment of eβ significantly increased the newly emerged weight and the ovarioles number in one ovary, as well as increased the expression level of Vg, hex70bandhex110 genes significantly in queen ovaries.In 1.0% treatment of eβ, the ovarioles number in one ovary and the expression of Vg and hex110 of queen were all increased significantly. Adding MS has no obvious effect on the newlyemerged weight, the ovarioles number and the expression level of the genes. But in 1.0% and 10.0% treatment of MS, the number of ovarioles decreased notably. The results showed that eβ added in queen rearing process can improve the queen quality to a certain extent.

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