| 莫文艳，王凤英，王国霞，胡俊茹，黄燕华,2020,黑水虻卵胚胎发育过程及在生产上的应用[J].环境昆虫学报,(3):701-706 |
| 黑水虻卵胚胎发育过程及在生产上的应用 |
| Study on the embryogenesis of black soldier fly (Hermetia illucens L) and its application in production |
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| DOI： |
| 中文关键词: 黑水虻 资源昆虫 虫卵 胚胎发育 镜检观察 生产应用 |
| 英文关键词:Black soldier fly resource insects eggs embryogenesis microscopic examination production application |
| 基金项目:广东省农业科学院学科团队建设项目（201614TD）；广东省农业科学院院长基金（201910） |
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| 中文摘要: |
| 黑水虻作为近年来广受关注的资源昆虫，其规模化应用的基础理论研究成为热点。本文对其胚胎发育过程进行研究，利用显微镜对资源昆虫黑水虻卵孵化发育过程进行观察并拍照，对胚胎各个时期的发育时间和形态进行记录。试验发现，在32℃下，黑水虻卵的发育历期为51 h。黑水虻的胚胎发育经过卵裂期、胚盘胚带形成期、原肠形成期、胚体分节期、体壁形成期、器官形成期6个典型时期。试验对孵化过程中的15个具体发育时期的特征和发育时间进行记录，形成了一套虫卵镜检技术应用于实际生产。在生产上，通过镜检技术对每一批次待孵化虫卵进行镜检观察，可初步判定该批次待孵化虫卵所处的发育阶段和死亡率，预估虫卵孵化时间和孵化率，为规模化稳定培育黑水虻苗种提供理论依据。 |
| 英文摘要: |
| As a resource insect that has received much attention in recent years, black soldier fly has become a hot topic in its basic theoretical research. This study was conducted to evaluate the embryogenesis of black soldier fly (Hermetia illucens L). The hatching process, development time and morphology of the black soldier fly eggs were recorded by a microscope. Results indicated that the embryogenesis of black soldier fly eggs experienced 51 h at 32℃. The embryogenesis including 6 typical phases: Cleavage stage, blastoderm embryo formation stage, primitive intestinal formation stage, embryo body segmentation stage, integument formation stage and organ formation stage. The characterization and development time of the 15 embryonic development stages were recoreded, forming a set of microscopic inspection techniques for eggs production. The microscopic examination technique can initially determine the development stage and mortality of the eggs, and estimate the incubation time and hatching rate. Moreover, results can provide guidance for the study of storage conditions of eggs, and provide a theoretical basis for the large scale stable cultivation of black soldier fly eggs |
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