| 高尚，蒋国芳，范冰冰,2020,基于微卫星标记探讨同域分布竹蝗间的基因渐渗[J].环境昆虫学报,(3):566-572 |
| 基于微卫星标记探讨同域分布竹蝗间的基因渐渗 |
| Gene introgreesion between two species of sympatric bamboo locusts based on microsatellite markers |
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| DOI： |
| 中文关键词: 竹蝗 微卫星标记 遗传多样性 基因渐渗 同域分布 |
| 英文关键词:Bamboo locusts microsatellites genetic diversity gene introgression sympatry |
| 基金项目:国家自然科基金(31572246) |
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| 中文摘要: |
| 竹蝗是我国竹林的重要害虫。同域分布物种间基因渐渗方面的研究较少，针对竹蝗属的尚未见报道。本文应用5对微卫星标记检测了安徽金寨县青脊竹蝗和黄脊竹蝗同域分布种群的遗传多样性、遗传结构及种群间的渐渗。结果表明，5对微卫星引物检测到的等位基因共60个，每个位点拥有等位基因10~13个，平均为12个；各位点的期望杂合度（He）范围为0.711~0.885,观测杂合度（Ho）均为1。每个位点都具有较高的多态性，平均多态信息含量（PIC）为0.799，说明这些位点都存在着丰富的遗传信息。在整体水平上，所有微卫星位点对之间为显著的连锁不平衡。同域分布的黄脊竹蝗和青脊竹蝗种群个体间发生了基因渐渗，且基因流从黄脊竹蝗转移到青脊竹蝗。最后，讨论并提出了对竹蝗属基因渐渗深入研究的建议。 |
| 英文摘要: |
| Bamboo locust is an important pest in bamboo forest in China Few studies have been carried out on gene introgreesion among sympatric species, and there is no report on bamboo locust. In this paper, five pairs of microsatellite markers were used to detect the genetic diversity, genetic structure and interpopulation introgreesion of the bamboo locusts Ceracris kiangsu and Ceracris nigricornis in Jinzhai County, Anhui Province. The results showed that there were 60 alleles detected by five pairs of microsatellite primers, each locus had 10 to 13 alleles, with an average of 12, the expected heterozygosity (He) range of each locus was 0.711~0.885, and the observed heterozygosity (Ho) range was 1. Each locus had a high polymorphism and the average polymorphic information content (PIC) was 0.799, indicating that these loci were rich in genetic information. At the overall level, there was a significant linkage disequilibrium among all microsatellite locus pairs. Gene introgreesion occurred between the populations of C. kiangsu and C. nigricornis, and the gene flow was transferred from C. kiangsu to C. nigricornis. Finally, some suggestions for further study on gene introgreesion of bamboo locust were discussed and put forward. |
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