| 陶海平，曹莉，韩日畴,2020,糖类和植物生长调节剂对冬虫夏草子实体人工培养的影响[J].环境昆虫学报,(2):274-281 |
| 糖类和植物生长调节剂对冬虫夏草子实体人工培养的影响 |
| Influence of medium components and plant growth regulators on artificial production of fruiting bodies of Ophiocordyceps sinensis |
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| DOI： |
| 中文关键词: 冬虫夏草菌 子实体 糖类 植物生长调节剂 |
| 英文关键词:Ophiocordyceps sinensis fruiting body sugars light plant growth regulator |
| 基金项目:广州市科技计划项目（201604020030, 201803010087）；国家自然科学基金项目（31900368）；广东省科学院青年引导专项（2019GDASYL-0105048） |
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| 中文摘要: |
| 为提高人工培养冬虫夏草子实体的产量，需要优化其培养参数。本实验测定培养基中糖类和植物生长调节剂对冬虫夏草子实体产量的影响。于大米小麦作为主要组分的培养基中接入冬虫夏草菌，在9~13℃下培养60 d，转入4℃培养。葡萄糖培养基中，冬虫夏草子实体干重是麦芽糖培养基中的7.6倍，出现菌丝和收获子实体的时间也比麦芽糖培养基中至少快2个月；蔗糖培养基中未获得子实体。不同种类和浓度植物生长调节剂对冬虫夏草子实体产量影响显著。于菌液中加入环磷腺苷、三十烷醇和玉米素的培养瓶均未发现菌丝生长。加入100 μg/mL 6-苄氨基腺嘌呤的培养瓶可见菌丝生长，但未见原基分化。转入4℃下6个月后，与对照相比，加入100 μg/mL吲哚乙酸、1 μg/mL和100 μg/mL吲哚丁酸、10 μg/mL赤霉素、1 μg/mL和10 μg/mL乙烯利，以及1 μg/mL 2,4-D的培养瓶中子实体干重均显著提高，其中加入1 μg/mL吲哚丁酸和1 μg/mL 2,4-D的培养瓶的子实体干重是对照的15倍。实验结果为优化冬虫夏草子实体人工培育提供了支撑。 |
| 英文摘要: |
| To increase the yields of fruiting bodies of Ophiocordyceps sinensis for artificial production, it is necessary to optimize the culture parameters. In this study, the influence of sugars, light and plant growth regulators on the yields of fruiting bodies of O. sinensis was determined. The media containing rice and wheat in 100 mL bottles were inoculated with fungal inocula and cultured at 9~13℃ for 60 d, and then at 4℃. The dry weight of fruiting bodies from the medium with glucose was 7.6 times more than that from the medium with maltose, and the mycelia and fruiting bodies from the medium with glucose appeared in two months ahead compared with the medium with maltose. No fruiting body was observed on the medium with sucrose. Significant differences were recorded among the dry weights of fruiting bodies from the media containing different plant growth regulators. No mycelia were observed from the media containing 3′,5′-cyclic AMP, triacontanol and zeatin Mycelia but no stroma were observed from the media with 100 μg/mL 6-benzyl adenine. After 6 months at 4℃, the dry weights of fruiting bodies from the media containing 100 μg/mL indole-3-acetic acid, 1 μg/M or 100 μg/mL indole-3-butyric acid, 10 μg/mL gibberellin acid, 1 μg/mL or 10 μg/mL ethephon, or 1 μg/mL 2,4-dichlorophenoxyacetic acid were significantly higher than that from the control (without any plant growth regulators). Especially, the dry weights of fruiting bodies from the media containing 1 μg/M indole-3-butyric acid or 1 μg/mL 2,4-dichlorophenoxyacetic acid were 15 times more than that from the control. The present results provide supports for optimized artificial production of fruiting bodies of O. sinensis. |
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