王宇飞,施腾飞,许圣云,Sawyer Burton,侯超,朱玉洁,余林生,2019,三种新烟碱农药对西方蜜蜂生存和生理的联合影响[J].环境昆虫学报,(3):612-618
三种新烟碱农药对西方蜜蜂生存和生理的联合影响
Combination effects of three neonicotinoid pesticides on physiology and survival of honey bees (Apis mellifera L.)
  
DOI:
中文关键词:  西方蜜蜂  新烟碱类农药  生存  生理  联合影响
英文关键词:Apis melliferaL.  neonicotinoid pesticides  survival  physiology  combination effect
基金项目:国家蜂产业技术体系建设专项(CARS-45-KXJ10)
作者单位
王宇飞,施腾飞,许圣云,Sawyer Burton,侯超,朱玉洁,余林生 1.安徽农业大学植物保护学院合肥 2300362.安徽农业大学生命科学学院合肥 2300363.安徽农业大学动物科技学院合肥 230036 
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中文摘要:
      为探究亚致死浓度的3种常用新烟碱农药噻虫嗪、吡虫啉和噻虫胺两两混合对西方蜜蜂生存以及乙酰胆碱酯酶和谷胱甘肽S-转移酶活性的联合影响,本研究通过饲喂管法检测这3种新烟碱农药对西方蜜蜂的经口性急性毒性。用各农药的 LD10 值作为处理剂量,检测 LD10 的各农药及其两两混合处理西方蜜蜂后48 h蜜蜂的死亡率,并检测各处理组蜜蜂乙酰胆碱酯酶和谷胱甘肽S-转移酶活性变化。3种农药两两互混后与单一农药相比均能显著增加西方蜜蜂的死亡率(P<0.05);噻虫嗪和吡虫啉以及噻虫嗪和噻虫胺联合用药均对蜜蜂毒性具有协同增效作用(Q>1.15)。所有农药处理组蜜蜂的乙酰胆碱酯酶活性均显著高于对照组(P<0.05),其中噻虫嗪与噻虫胺混合组乙酰胆碱酯酶的活性最高;噻虫嗪和吡虫啉以及噻虫嗪和噻虫胺两两互混后与单一农药处理组相比均能显著增加蜜蜂乙酰胆碱酯酶活性(P<0.05)。除噻虫嗪与噻虫胺互混组对蜜蜂谷胱甘肽S-转移酶活性具有明显的抑制作用(P<0.05),其他农药组谷胱甘肽S-转移酶活性均没有明显变化。新烟碱类农药噻虫嗪和吡虫啉以及噻虫嗪和噻虫胺互混处理组能够协同增加对蜜蜂的毒性,而且农药混合与单一农药处理相比能引起蜜蜂不同的生理变化。本研究利于更深入了解和探究混合农药对蜜蜂健康的影响,并为保护蜜蜂提供一定理论基础。
英文摘要:
      To investigate combination effects of three common neonicotinoid pesticides thiamethoxam, imidacloprid and clothianidin on the activities of acetycholinesterase (AChE) and glutathione S.transferases (GSTs), and the survival in honey bees (Apis mellifera L.). The acute toxicity of three neonicotinoids pesticides to honey bees was tested by “oral feeling tube” method; The mortalities, AChE and GSTs activities of honey bees was measure at 48 h after bees were exposed to LD10 of thiamethoxam, imidacloprid and clothianidin, and binary mixtures of these pesticides.The mortalities were significantly increased by binary mixtures of three pesticides (P<0.05). The synergistic effect of thiamethoxam and imidacloprid, or thiamethoxam and clothianidin could increase the toxicity to bees(Q>1.15). The activities of AChE in pesticide treatment groups were all significantly higher than control (P<0.05). Binary mixtures of thiamethoxam and imidacloprid, or thiamethoxam and clothianidin could significantly increased the activities of AChE (P<0.05). The activities of GSTs were distinctly inhibited by the mixture of thiamethoxam and clothianidin (P<0.05); however, there were no significant differences in the activities of GSTs in other pesticide groups.Combined toxicity was detected from mixtures of thiamethoxam and imidacloprid, or thiamethoxam and clothianidin showed synergistic interaction.Binary mixtures of neonicotinoids showed different physiology changes than single neonicotinoids in honeybees.These results are important for exploring impact of pesticides on the health of honey bees and provide theoretical basis in honey bees protection.
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