| 陈文锋，谢甲钰，王丹凤，杨广,2019,蚤蝇的昼夜活动节律及睡眠行为研究[J].环境昆虫学报,(3):435-442 |
| 蚤蝇的昼夜活动节律及睡眠行为研究 |
| Study on the locomotor circadian rhythm and sleep behaviors of Megaselia scalaris |
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| DOI： |
| 中文关键词: 蚤蝇 昼夜活动节律 睡眠 |
| 英文关键词:Megaselia scalaris locomotor circadian rhythm sleep |
| 基金项目:国家自然科学基金（31601894）；福建省自然科学基金（2017J0106） |
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| 摘要点击次数: 1929 |
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| 中文摘要: |
| 蚤蝇是重要的法医昆虫，同时是实验室中遗传、发育和生物测定等研究的重要对象。然而，蚤蝇的昼夜活动节律和睡眠行为及其在脑部的神经网络目前还不清晰。本文通过捕获本地蚤蝇并对其进行分子鉴定，研究了蚤蝇的昼夜活动节律和睡眠行为，同时表征了蚤蝇脑部核心钟神经元和多巴胺神经元。结果表明：蚤蝇在12 h光照∶12 h黑暗（12L∶12D）条件下不存在对开灯前或关灯前的活动预期，其双峰活动模式是对开关灯的光反应行为。在全黑暗（DD）条件下蚤蝇内源活动周期接近24 h。黑腹果蝇神经肽PDF抗体免疫显示蚤蝇脑部核心钟神经元4~5个，不像黑腹果蝇一样存在明显的神经轴突。在睡眠行为上，蚤蝇雄虫和雌虫在整体活动强度、睡眠节律模式、总睡眠上均没有明显差异。相反，雄虫总睡眠次数和晚上睡眠次数低于雌虫，而总睡眠持续时间、晚上睡眠持续时间、总入睡时间和晚上入睡时间高于雌虫。此外，影响睡眠的重要多巴胺神经元在蚤蝇脑部的分布与黑腹果蝇类似。 |
| 英文摘要: |
| Megaselia scalaris is an important forensic insect and be used for genetics, development and bioassay researches in the laboratory.However, as important behaviors of insects, the locomotor circadian rhythm and sleep behaviors and their brain neural networks are still unclear in the M.scalaris.In this paper, we captured the local M.scalaris and molecularly identified them, and then studied their locomotor circadian rhythm and sleep behaviors. Meanwhile, the core clock neurons and dopamine neurons in the brain of theM.scalaris was characterized.Results are as follows: Under the 12 h light∶12 h dark (12 L∶12 D) condition, there was no anticipation of activity before light on or light off in the M.scalaris, and its bimodal activity pattern was the photoreaction behavior of the switch lamp. Under the full darkness condition (DD), the endogenous activity period of the M.scalariswas close to 24 h.Immunization using the neuropeptide PDF antibody from the Drosophila melanogaster only show 4~5 core clock neurons in the brain of M.scalaris, and there were no obvious axons like that in the D.melanogaster.In terms of sleep behavior, there was no significant difference in activity intensity, sleep rhythm pattern and total sleep between males and females of the M scalaris.In contrast, males had fewer total or night number of sleep than females, while total sleep duration, nighttime sleep duration, total sleep latency and nighttime sleep latency in males were higher than in females.Immunization using the tyrosine hydroxylase antibody from theD.melanogastershowed that the distribution of important sleep related dopamine neurons in the brain of M.scalaris was similar to that of D.melanogaster. |
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