| 张晓宇，赵晓峰，许益镌，程代凤，陆永跃,2018,红火蚁共生链霉菌Streptomyces sp. DF-5的分离培养及其发酵液对植物病原真菌的抑制活性研究[J].环境昆虫学报,40(4):917-924 |
| 红火蚁共生链霉菌Streptomyces sp. DF-5的分离培养及其发酵液对植物病原真菌的抑制活性研究 |
| Identification and culture ofStreptomycessp. DF-5 isolated from Solenopsis invictaand the antifungal activity of its fermentation broth to plant pathogenic fungi |
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| DOI： |
| 中文关键词: 红火蚁 链霉菌DF-5 生长特性 抑真菌活性 |
| 英文关键词:Solenopsis invicta Buren Streptomycessp. DF-5 growth characteristic antifungal activity |
| 基金项目:国家重点研发计划项目（2016YFC1201200）；广东省科技发展专项资金项目（2017B020202009） |
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| 中文摘要: |
| 自入侵中国以来红火蚁Solenopsis invicta Buren受到广泛关注，明确与该蚁共生或者伴生的微生种类及其生物学功能具有重要意义。本研究从红火蚁工蚁体中筛选出一个共生菌株，观察、分析了其生长、生理生化特性和16S rRNA同源序列特征，应用菌丝生长速率法测定了该菌株发酵液滤液对3种植物病原真菌的抑菌作用。鉴定结果表明该菌株属于链霉菌属Streptomyces，命名为Streptomyces sp. DF-5，其发酵液滤液对常见植物病原真菌如荔枝霜疫霉病菌Peronophythora litchii、香蕉枯萎病菌Fusarium oxysporum f. sp.cubense和稻瘟病菌Magnaporthe oryzae具有较好的抑制效果，抑制率均在70%以上，其中对荔枝霜疫霉病菌的抑制效果高达94.5%。本研究可为利用红火蚁共生菌开发新型生物农药提供参考。 |
| 英文摘要: |
| Since its invading China, red imported fire ant Solenopsis invicta Buren has been widely concerned, and it is of significance to identify the symbiont species with the fire ant and their biological functions. In this study, a symbiotic strain was screened from the fire ant workers, and the growth, physiological and biochemical characteristics and the 16S rRNA homologous sequence were observed and analyzed. Inhibitory activity of the filtrate of the fermentation liquid of the strain on 3 plant pathogenic fungi was measured by the mycelial growth rate method. The results showed that the strain was Streptomycessp. DF-5, and the filtrate of fermentation liquid had good inhibitory effect on the plant pathogenic fungi including Peronophythora litchi, Fusarium oxysporum f. sp.cubense, Magnaporthe oryzae, and the inhibitory rates were all above 70% with the highest (94.5%) onP. litchii. This study could provide a reference for developing new biological pesticides from the symbionts of red imported fire ant. |
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