| 董彬，叶小芳，向敏，扈鸿霞，于非，邓露，朱娟，季荣，王晗,2018,短时高温暴露下东亚飞蝗雌虫血淋巴蛋白表达分析及质谱鉴定[J].环境昆虫学报,40(4):901-907 |
| 短时高温暴露下东亚飞蝗雌虫血淋巴蛋白表达分析及质谱鉴定 |
| Expression analysis and identification of proteins in hemolymph of Locusta migratoria manilensis Meyen (Orthoptera: Acrididae) under short-term exposure to high temperature |
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| DOI： |
| 中文关键词: 高温 血淋巴 蛋白定量 蛋白鉴定 东亚飞蝗 |
| 英文关键词:High temperature hemolymph quantitative analysis of the protein protein identification Locusta migratoria |
| 基金项目:新疆特殊环境物种多样性应用与调控实验室开放课题（XJTSWZ-2017-01）;自治区高校科研计划项目（XJEDU2017M023）;国家自然科学基金（31260104）;科技部国际合作专项（2015DFR30290）;天山创新团队计划项目 |
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| 中文摘要: |
| 为研究短时高温对东亚飞蝗Locusta migratoria manilensis Meyen血淋巴蛋白的影响，采用Bradford法、SDS-PAGE电泳和质谱等方法，对东亚飞蝗雌虫血淋巴样品进行检测。结果表明：短时高温对血淋巴蛋白含量有显著影响（P<0.01），36℃-42℃范围内，随温度升高，血淋巴蛋白浓度亦升高，其中39℃、42℃处理组与对照组差异显著（P＜0.01）；短时高温对血淋巴蛋白种类存在一定影响，对照组雌虫血淋巴中存在11种蛋白，高温处理后，4种蛋白含量逐渐增加，6种蛋白含量没有明显变化，1种蛋白消失；经质谱检测，鉴定了5种蛋白，分别为载脂蛋白前体、酚氧化酶原、2个储存蛋白和19 kDa血淋巴蛋白，另外6条蛋白未被鉴定。推测载脂蛋白前体、酚氧化酶原、储存蛋白在东亚飞蝗应对高温胁迫过程中发挥重要作用。 |
| 英文摘要: |
| In order to study the effect of short-term exposure to high temperature on proteins in hemolymph of Locusta migratoria manilensis Meyen, proteins in hemolymph of female adult locust were detected by Bradford assay, SDS-PAGE electrophoresis and MALDI-TOF-TOF/MS analysis. The results showed that short-term exposure to high temperature did a significant effect on concentration of protein in hemolymph (P<0.01). The concentration was increased with increasing temperature in the range of 36℃-42℃. There had significant increase on concentration between the treatment group (39℃,42℃)and the control (P＜0.01). Short-term exposure to high temperature affected types of proteins in hemolymph. There had 11 types of proteins in hemolymph in the control. With increasing temperature, the concentration of 4 types of proteins were increased while 6 types of proteins showed no changed. Under different temperature treatments, 1 type of proteins was disappeared. By MALDI-TOF-TOF/MS analysis, 5 types of proteins were identified as apolipophorin precursor protein, pro-phenoloxidase 2, hexamerin-like protein 1, hexamerin-like protein 2 and basic 19 kDa hemolymph protein. The other 6 types of proteins were not identified. Apolipophorin precursor protein, pro-phenoloxidase, hexamerin protein may play pivotal roles in response to high temperature stress. |
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