《环境昆虫学报》

作者	单位
马光昌，王晓妮，牛黎明，龚治，温海波，金启安，彭正强	中国热带农业科学院环境与植物保护研究所，海口 571101

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中文摘要:

新菠萝灰粉蚧Dysmicoccus neobrevipes（Beardsley）是一种严重为害剑麻、番荔枝等经济作物的入侵性害虫。由于粉蚧类昆虫体型较小，且外部形态十分相似，难以达到快速准确识别。本文利用分子标记技术，研究了新菠萝灰粉蚧的快速鉴定方法。通过对新菠萝灰粉蚧、扶桑绵粉蚧、双条拂粉蚧、杰克贝尔氏粉蚧、大洋臀纹粉蚧、木槿曼粉蚧、甘蔗粉蚧和木瓜粉蚧等8种粉蚧的序列进行分析，针对新菠萝灰粉蚧设计了1对特异性引物，其扩增片段大小为552 bp（GenBank登录号为MF 363108），该引物对其它7种粉蚧没有扩增能力。该引物不仅对新菠萝灰粉蚧成虫具有扩增效能，对1龄若虫、2龄若虫和3龄若虫都具有同样的扩增能力，其最低检出阈值为437.5 pg/μL。该技术的建立，实现了在剑麻等苗木调运过程中对新菠萝灰粉蚧的快速准确鉴定，同时对新菠萝灰粉蚧的检疫和监测也具有重要的意义。

英文摘要:

Dysmicoccus neobrevipes(Beardsley) is an invasive pest, which tremendously harms economic crops including Agave sisalana and Annona squamosa. It is very hard to rapidly and accurately distinguishing mealybugs because of their small size and similarity in external morphology. In this study, we had developed a method for the rapid differentiation of D.neobrevipesby molecular marker technique. Sequences of D.neobrevipes、Phenacoccus solenopsis Tinsley、Ferrisia virgata Cockerell、Pseudococcus jackbeardsleyi、Planococcus minor Maskell、Maconellicoccus hirsutus、Saccharicoccus sacchari Cockerell and Paracoccus marginatus wereanalysed. A pair of specific primers which is specific to D.neobrevipes was designed. A single 552 bp (GenBank accession number: MF 363108) fragment was amplified using the specific primers only in D.neobrevipes. No fragments were amplified using the specific primers in other mealybugs. The amplification ability of specific primer was proved to be capable for not only adults but also 1st, 2nd and 3rd instar of D.neobrevipes. Moreover, the assays was capable of detecting 437.5 pg/μL. This technique should be useful tool for pest rapid diagnosis, quarantine and monitoring during transportation of seedlings like A.sisalana.

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