谷小红,蔡普默,杨晴阳,季清娥,陈家骅,2017,阿里山潜蝇茧蜂对橘小实蝇危害诱导水果挥发物的行为反应及主要成分分析[J].环境昆虫学报,(4):820-829 |
阿里山潜蝇茧蜂对橘小实蝇危害诱导水果挥发物的行为反应及主要成分分析 |
Behavioral responses of Fopius arisanus (Sonan)(Hymenoptera: Braconidae)to volatiles from fruits infested by Bactrocera dorsalis(Hendel)(Diptera: Tephritidae)and analysis of volatile components |
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DOI: |
中文关键词: 阿里山潜蝇茧蜂 桔小实蝇 行为反应 挥发物 |
英文关键词:Fopius arisanus Sonan Bactrocera dorsalis Hendel behavioral responses volatiles |
基金项目:国家教育部博士点专项科研基金(K41110003);福建省自然科学基金(2016J01114) |
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中文摘要: |
为明确寄主植物挥发物在阿里山潜蝇茧蜂Fopius arisanus Sonan生境定位,寄主或食物搜寻等过程中的作用,采用Y型嗅觉仪测定了阿里山潜蝇茧蜂性成熟雌蜂对健康、虫害诱导及人为损伤的橙子、香蕉、西红柿的行为反应,并用气相色谱-质谱联用仪分析鉴定受染前后的番石榴、橙子、香蕉和番茄挥发物成分。结果表明:健康的、橘小实蝇危害和机械损害的香蕉、橙子对阿里山潜蝇茧蜂性成熟雌蜂均产生显著的引诱作用,受染橙子引诱到寄生蜂的数量显著高于健康橙子,其他组对于水果气味的选择并无显著性差异,并且阿里山潜蝇茧蜂雌成蜂对虫害诱导水果的选择反应与为害时间密切相关。4种不同水果挥发物成分种类多样,相对含量及其比例差异较大。橘小实蝇为害后诱导水果新产生了乙酸叶醇酯、反-法呢烯、β-罗勒烯、苯甲醛、顺-罗汉柏烯、柏木脑、辛酸乙酯、辛酸甲酯、十四烷、十五烷和二十烷。芳樟醇和β-月桂烯在受染橙子的释放具有滞后性,分别在橙子受染后第2天和第3天才被检测到,并且相对含量逐渐上升。上述结果为研究水果挥发物调控阿里山潜蝇茧蜂的行为反应,提高其生物防治效果奠定基础。 |
英文摘要: |
In order to investigate the roles of volatiles from host plants in the process of Fopius arisanus Sonan targeting habitats, searching for host or food, the behavioral responses of sexually mature F.arisanus females to intact fruits, fruits infested by Bactrocera dorsalis Hendel and damaged mechanically fruits of oranges, bananas and tomatoes were measured by Y-tube olfactometer, and the volatiles from intact fruits and fruits infested by B.dorsalis of guavas, oranges, bananas and tomatoes were identified by gas chromatography-mass spectrometry (GC-MS). The results showed that the F.arisanus sexually mature females were significantly attracted by intact fruits, fly-infected and damaged mechanically fruits of bananas and oranges, the numbers of F.arisanus mature females attracted by infected orange were significantly more than intact oranges, and there were no significant differences in other groups. The selective responses of F. arisanus sexually mature females to infected fruits were closely related to the time of fruit fly infecting. Volatile compounds from four different fruits were diversity, their relative contents and proportion were quite different. Fruits infected by B.dorsalis newly released Leaf acetate, trans-Farnesol, beta-Ocimene, Benzaldehyde, cis-Thujopsene, Cedrol, Octanoate ethyl, Octanoate methyl, Tetradecane, Pentadecane. The delayed release of Linalool and beta-Myrcene in infected oranges were observed. The above results laid a necessary foundation for further research of volatiles regulating the behavioral responses of F.arisanus and to improve its biocontrol effectiveness. |
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