| 王雪丽，李静静，黄翠虹，闫凤鸣,2017,L-阿拉伯糖对B型和Q型烟粉虱刺探的影响—膜饲喂评价技术的优化[J].环境昆虫学报,(4):770-778 |
| L-阿拉伯糖对B型和Q型烟粉虱刺探的影响—膜饲喂评价技术的优化 |
| Effects of L-Arabinose on probing behavior of Bemisia tabaci B and Q biotypes—Optimizing the membrane feeding evaluation method |
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| DOI： |
| 中文关键词: 烟粉虱 L-阿拉伯糖 刺孔 刺探行为 人工饲料 |
| 英文关键词:Bemisia tabaci L-Arabinose piercing hole probing behavior artificial diet |
| 基金项目:国家自然科学基金（31471776） |
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| 中文摘要: |
| 本文以观察人工饲料膜上刺孔直径及数量评价L-阿拉伯糖对B型和Q型烟粉虱刺探及取食行为为例，用人工饲喂的方法，从饲料的最佳蔗糖浓度、L-阿拉伯糖最佳添加浓度、实验最佳实验时间3个方面对膜喂饲评价技术进行优化，为刺吸式昆虫取食行为的基础研究提供简单可行的方法。结果表明：15%蔗糖最适合烟粉虱的饲喂实验，5% L-阿拉伯糖是合适的添加浓度，12 h是检查效果的最佳时间；取食添加5% L-阿拉伯糖的饲料后，B型烟粉虱在人工饲料囊上的刺探所形成的的大孔、中孔及小孔的数量均显著低于对照，而Q型烟粉虱刺探所形成的的大孔、中孔及小孔数量均显著高于对照。即L-阿拉伯糖抑制了B型烟粉虱的刺探和取食行为，却提高了Q型烟粉虱的刺探和取食行为，因此，5% L-阿拉伯糖对两种生物型烟粉虱可能存在不同作用机制。以15%蔗糖的液体人工饲料膜上的刺孔直径及数量来评价生物活性物质对烟粉虱取食行为影响效果的方法是可行的。 |
| 英文摘要: |
| This paper took the observation of diameters and amount of probing holes on the artificial diet capsules to evaluate the effects of L-Arabinose on the probing and feeding behavior of Bemisia tabaci for instance, according to the method of artifical feeding. The membrance feeding evaluation technique was optimized from three aspects: The optimum sucrose concentration, the optimum L-Arabinose adding concentration and the optimum experimental time, which provided a simple and feasible method for the basic research of feeding behavior of piercing-sucking insect pests. The results showed that 15% sucrose was the most suitable concentration for the growth of whiteflies, 5% L-Arabinose is the suitable adding concentration, the twelfth hour is the best time to check the result; compared the diameters and amount of probing holes on the artificial diet capsule produced by B. tabaci B After fed on 5% L -Arabinose, it found out that the total number of probing holes as well as big holes, middle holes and small holes produced by B. tabaci B were significantly lower than the control; However, the total number of probing holes as well as big holes, middle holes and small holes produced by B. tabaci Q were significantly higher than the control. That was to say that probing and feeding behavior were inhibited by L-Arabinose in B biotype, but were stimulated in Q biotype. So, 5% L-Arabinose may have different mechanism in B. tabaci B and B. tabaci Q. It is feasible to evaluate the effect of bioactive chemicals on the feeding behavior of B.tabaci by diameters and amount of probing holes on the artificial diet capsules with 15% sucrose. |
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