| 曹伟平，宋健，赵建江，冯书亮，杜立新,2017,球孢白僵菌HFW-05对烟粉虱的致病力及组织病理影响[J].环境昆虫学报,(4):762-769 |
| 球孢白僵菌HFW-05对烟粉虱的致病力及组织病理影响 |
| Pathogenicity and histopathological affection of Beauveria bassiana HFW-05 on Bemisia tabacinymphs |
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| DOI： |
| 中文关键词: 球孢白僵菌 烟粉虱 致病力 组织病理变化 |
| 英文关键词:Beauveria bassiana Bemisia tabaci pathogenicity histopathological changes |
| 基金项目:河北省科技计划项目（13226510D）；河北省农科院研究与发展项目（A2015120102）；河北省财政项目(F16C10003) |
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| 中文摘要: |
| 环境温湿度是影响白僵菌菌株致病力的关键因素，本文测定了不同温、湿度下白僵菌Beauveria bassiana HFW-05对烟粉虱Bemisia tabaci若虫及卵的致病力，同时观察了烟粉虱感病若虫的组织病理变化。结果表明，各浓度下烟粉虱若虫的校正死亡率随时间变化曲线基本一致，接种3-4 d时出现死亡高峰，白僵菌HFW-05对烟粉虱1龄、2龄、3龄若虫的LC50分别为5.94×10^4、1.06×10^5、5.08×10^5孢子/mL，LT50分别为2.54 d、2.68 d、3.18 d；白僵菌HFW-05对卵的致病力很低，1×10^8孢子/mL接种浓度下，卵的6 d感染率仅为24.9%。在25℃、30℃下，HFW-05对烟粉虱若虫表现出最强的致病力，高于或低于此温度时，烟粉虱若虫死亡速度减慢，死亡率降低。相对湿度发生变化时，烟粉虱若虫死亡速度和死亡率明显不同，相对湿度高于90%时，烟粉虱若虫死亡速度最快，死亡率最高，相对湿度介于70%-90%时，白僵菌HFW-05对烟粉虱若虫的致病力无明显差异，但校正死亡率显著低于环境湿度大于90%的处理，相对湿度低于70%时,烟粉虱死亡率也达到60%左右。组织病理学观察显示尾部舌状突是HFW-05侵染烟粉虱若虫的关键部位。研究结果表明白僵菌HFW-05在烟粉虱的生物防治中有较大的潜力。 |
| 英文摘要: |
| Temperature and humidity are key factors to affect the pathogenicity of Beauveria bassiana HFW-05.The pathogenicity of B. bassiana to Bemisia tabaci nymphs under different temperatures and humidities were studied in the laboratory. The histopathological changes of B. tabaci nymphs infected by strain HFW-05 also were observed. The corrected mortality of each period of B. tabaci nymphs treated by strain HFW-05 showed the same curves. The infection peak day of different instar nymphs appeared on the 3rd-4th day after inoculation. The LC50of the first to the third instar nymphs were 5.94×10^4，1.06×10^5 and 5.08×10^5conidia/mL , respectively. The LT50were 2.54 d，2.68 d and 3.18 d, respectively. It showed very low pathogenic activity to the egg which infection rate was 24.9% after 6 d inoculation with 1×10^8 conidia/mL concentration. Strain HFW-05 showed the highest pathoginicity to B. tabaci nymphs at 25℃ and 30℃. The pathoginicity of HFW-05 decreased when the temperature ＜25℃ or ＞30℃. The mortalities of the B. tabaci nymphs under different humidities showed significant differences. The nymphs died most quickly and the mortality was the highest under above 90% relative humidity, while the mortality decreased drastically under 70%-90% relative humidity. The mortality was about 60% under below 70% relative humidity. The conical lingula of B. tabaci nymphs was the most important invasive part. B. bassiana HFW-05 has the potential to be used for biological control against B. tabaci. |
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