| 庞倩，王莹，王康，张文文，吉挺*,2017,不同移虫日龄蜂王卵巢中hexamerin110、hexamerin70b的差异表达分析[J].环境昆虫学报,39(1):62-67 |
| 不同移虫日龄蜂王卵巢中hexamerin110、hexamerin70b的差异表达分析 |
| Analysis on differential expression of hexamerin 110 and hexamerin70b in the ovaries of queens reared from different instar of the grafted larvae |
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| DOI： |
| 中文关键词: 移虫日龄 卵巢 hexamerin110 hexamerin70b 级型分化 |
| 英文关键词:Instar of the grafted larvae ovary hexamerin110 hexamerin70b caste differentiation |
| 基金项目:国家自然科学基金（31502020，31172272）；现代农业产业技术体系（CARS-45-SYZ6） |
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| 中文摘要: |
| 为探究不同移虫日龄的西方蜜蜂蜂王卵巢中hexamerin110、hexamerin70b的表达及其调控机理，以β-actin为内参基因，运用qRT-PCR技术检测不同移虫日龄蜂王卵巢中hexamerin110、hexamerin70b mRNA的表达水平，用Western blot方法检测hexamerin110、hexamerin70b蛋白的表达。结果显示，hexamerin110、hexamerin70无论是在mRNA水平还是蛋白水平，随着移虫日龄的增加，其表达量均呈降低的趋势。1日龄移虫培育的蜂王卵巢hexamerin110基因表达量显著高于其他组（P＜0.05，1日龄移虫培育的蜂王卵巢的hexamerin70b基因的表达量极显著高于其他组（P＜0.01。Hexamerin110、hexamerin70b在1日龄移虫发育的蜂王卵巢中的表达水平最高，推测其可能与蜂王生殖发育以及级型分化有关。 |
| 英文摘要: |
| To explore the expression and molecular regulation mechanism of hexamerin110 and hexamerin70b in the ovaries of queens reared from different instar of the grafted larvae, mRNA and protein expression of hexamerin110 and hexamerin70b were assessed by qRT-PCR and Western blot. The results showed that the expression levels of thehexamerin110 and hexamerin70b decreased with the increasing instar of the grafted larvae, the expression levels of the hexamerin110 in ovaries of queens reared from 1 day instar of the grafted larvae were significantly higher than others (P<0.05). Meanwhile, the expression levels of the hexamerin70b in ovaries of queens reared from 1 day instar of the grafted larvae were extremely significantly higher than others (P<0.01). The expression levels of the hexamerin110 and hexamerin70b of 1 day instar of the grafted larvae are the highest, it is speculated that hexamerin110 and hexamerin70b may be associated with queen reproductive development and caste differentiation. |
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