| 高欢欢，翟一凡，徐楠，覃冬云，葛温伯，于毅*,2016,五种细菌侵染后韭菜迟眼蕈蚊体内防御反应的研究[J].环境昆虫学报,(3):582-588 |
| 五种细菌侵染后韭菜迟眼蕈蚊体内防御反应的研究 |
| Defensive respondes of Bradysia odoriphaga to inoculation of five species of bacteria |
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| DOI： |
| 中文关键词: 韭蛆 细菌 侵染 保护酶 |
| 英文关键词:Bradysia odoriphaga bacteria infection defensive enzyme |
| 基金项目:社会公益性研究项目（201303027） |
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| 中文摘要: |
| 为了解韭蛆体内5种细菌对韭菜迟眼蕈蚊Bradysia odoriphaga的影响，本研究将解淀粉芽孢杆菌Bacillus amyloliquefaciens、居泉沙雷氏菌Serratia fonticola、恶臭假单胞杆菌Pseudomonas putida、枯草芽胞杆菌Bacillus subtilis和节杆菌Arthrobacter nitroguajacolicus 5种细菌通过浸泡和针刺两种方式对韭蛆进行侵染，观察韭蛆存活率、蛋白含量以及3种抗氧化酶活性的变化规律。结果显示，与浸泡接种细菌相比，针刺接种细菌后，韭蛆整体的存活率和SOD 酶活性下降，而蛋白含量、POD活性和CAT活性上升。其中，居泉沙雷氏菌在针刺侵染后对韭蛆表现出致病性。因此，机械损伤对于细菌的侵染具有重要的作用，韭蛆则可通过改变抗氧化酶的活性来防御细菌的侵染。本研究为探索韭蛆与体内共生微生物之间的关系提供重要的基础，为韭蛆的共生防治提供指导。 |
| 英文摘要: |
| In order to understand the effect of bacteria onBradysia odoriphaga, the survival rate, protein content and three oxidative enzymes of B.odoriphaga were examined after inoculation by five species of bacteria according to two inoculation methods (soak and wound). The bacteria were: Bacillus amyloliquefaciens, Serratia fonticola, Pseudomonas putida, Bacillus subtilis andArthrobacter nitroguajacolicus. The result showed that, compared to soak inoculation by bacteria, survival rate and SOD activity of B.odoriphagadecreased, and protein content, activity of POD and CAT increased after wound inoculation. The negative influence on B.odoriphaga was found after wound inoculation by S.fonticola. It was concluded that, could be affected by the mechanical injury could contributed to the infection of bacteria to B.odoriphaga. B. odoriphaga could produced defensive responds to the inoculation of bacteria through changing activity of oxidative enzyme. This study provided the theoretical foundation for exploring the coevolution relationships between B.odoriphaga and microorganisms. And we hope that it will contribute to guiding for the symbiotic control ofB.odoriphaga |
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