| 牟星,王进军*,2016,3种抑制剂对褐色橘蚜乙酰胆碱酯酶活性的影响[J].环境昆虫学报,(2):401-407 |
| 3种抑制剂对褐色橘蚜乙酰胆碱酯酶活性的影响 |
| Effect of three insecticides on acetylcholinesterase of Toxoptera citricida in vitro |
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| DOI： |
| 中文关键词: 褐色橘蚜 乙酰胆碱酯酶 动力学 离体抑制 |
| 英文关键词:Toxoptera citricida AChE kinetics in vitro inhibition |
| 基金项目:国家级大学生创新创业训练计划（201210635036）；重庆市自然科学重点基金（CSTC2013jjB0176） |
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| 中文摘要: |
| 乙酰胆碱酯酶（Acetylcholinesterase, AChE; EC3.1.1.7）作为有机磷和氨基甲酸酯类杀虫剂的作用靶标，一直是昆虫毒理学领域研究的热点。本研究采用微量滴度酶标板法研究了褐色橘蚜Toxoptera citricida不同发育阶段AChE的生化特性及个体活性频率，离体条件下分析了3种药剂对AChE的影响。结果表明，无翅成蚜对底物碘化硫代乙酰胆碱亲和力显著高于若蚜；就个体活性分布频率而言，25%-30%的无翅成蚜分布在9.5-10.5 nmol/(min·insect)区间，而50%-55%的若蚜分布在2-3 nmol/(min·insect)区间；离体条件下，毒扁豆碱、残杀威、异丙威等3种杀虫剂对褐色橘蚜AChE有显著的抑制作用，与成蚜相比，若蚜AChE对3种药剂更敏感，而毒扁豆碱对褐色橘蚜无翅成蚜与若蚜AChE的抑制作用最好。本研究表明褐色橘蚜成蚜与若蚜生化特性及对药剂的敏感性存在显著差异。 |
| 英文摘要: |
| Acetylcholinesterase (AChE) plays an important role in the nervous impulse of natural speciesThe biochemical and toxicological characteristics of AChE from Toxoptera citricida wingless adult and nymph were studied in the laboratory applying microplate reader assay. The results showed that AChE from wingless adult aphid on substrate affinity was significantly higher than that of nymphs. As for the individual distribution of AChE activity in frequency, 25%-30% of wingless adults are distributed in the range of 9.5-10.5 nmol/(min·insect), 50%-55% of nymphs are focused on the range of 2-3 nmol/(min·insect). Besides, all three kinds of inhibitors significantly inhibited AChE from T.citricida wingless adults and nymphs. Compared to the counterparts of wingless adults, AChE from nymphs is more sensitive to insecticides. Among the insecticides, serine exhibited best inhibition effect on the activity of AChE from T.citricida. The current research showed that biochemical properties and the insecticide sensitivity of AChE from T.citricida wingless adult and nymph were significantly different. |
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