《环境昆虫学报》

为明确异色瓢虫 Harmonia axyridis （Pallas）在取食日本龟蜡蚧 Ceroplastes japonicus Green过程中虫体挥发物的作用，本研究采用Y型嗅觉仪测定了异色瓢虫对日本龟蜡蚧的趋性行为反应，并利用顶空固相微萃取和气质联用（HS-SPME-GC/MS）对日本龟蜡蚧（卵、若虫、成虫）的挥发性气味进行分析鉴定。结果表明：异色瓢虫对日本龟蜡蚧各龄期均有趋性，其中对成虫的趋性达到极显著水平（ P＜0.01）。在日本龟蜡蚧虫体挥发性成分中，共检出10种烷烃类、8种烯烃类、9种醇类等47种组分。选取其中两种含量较大的组份1-辛烯-3-醇和1R-α-蒎烯进行单组份趋性试验，结果表明1-辛烯-3-醇在浓度为10-4 g/mL和10-5g/mL、1R-α-蒎烯在浓度为10 -5 g/mL和 10 -6 g/mL 时对异色瓢虫具有明显吸引效应，其中10-4g/mL的1-辛烯-3-醇和10 -5 g/mL的1R-α-蒎烯引诱率分别达到73.65%和76.41%，差异达到极显著水平（P＜0.01）。上述结果为进一步研究异色瓢虫的引诱剂奠定了基础。

英文摘要:

To clear the function of the body volatile of Ceroplastes japonicus Green in the foraging of Harmonia axyridis (Pallas), selection response of H.axyridis to C.japonicus was investigated by using the Y-olfactory instrument, and the volatile components of the scale insect(egg, nymph, adult)were studied by headspace solid phase micro-extraction and gas chromatography-mass spectrometry（HS-SPME-GC/MS）. The results showed that H.axyridis showed obvious selection to C.japonicus in different instars, and the selective effect to adult reached significantly different level (P＜0.01). A total of 47 compounds were analyzed and identified, including 10 alkanes, 8 alkenes, 9 alcohols and so on. Furthermore, the two compounds (1-Octen-3-l and 1R- alpha -Pinene) were selected from the volatile to examine their attraction power to the ladybug. The result indicated that 1-Octen-3-ol displayed an obvious attractiveness to H.axyridis at concentration of 10 -4 g/mL and 10 -5 g/mL, while 1R- alpha -Pinene did the same role in 10-5 g/mL and 10 -6 g/mLAnd the tendency percentages to 1-Octen-3-ol at concentration of 10 -4 g/mL and 1R- alpha -Pinene at concentration of 10 -5 g/mL were 73.65%and 76.41%, which reached significantly different level (P＜0.01). The results laid a foundation for further research on the attractant of H.axyridis.

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