| 黄翠虹1，游秀峰2，王珏2，李为争2，闫凤鸣2*,2015,菜粉蝶对十字花科植物挥发物的触角电位反应及引诱剂配方的大田诱捕试验[J].环境昆虫学报,37(6):1219-1226 |
| 菜粉蝶对十字花科植物挥发物的触角电位反应及引诱剂配方的大田诱捕试验 |
| Antennal responses of the cabbage butterfly, Pieris rapae, to some common volatiles of Brassicaceae and a long-term field trapping based on uniform mixture design |
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| DOI： |
| 中文关键词: 菜粉蝶 花香气味 配方均匀设计 |
| 英文关键词:Pireris rapae floral scents electroantennogram mixture of uniform design field trapping |
| 基金项目:农业部公益性行业科研专项（201203036） |
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| 中文摘要: |
| 通过诱杀成虫控制其后代幼虫的种群，是进行害虫控制的新思路。为了提供具有实用价值的引诱剂对菜粉蝶进行有效的化学生态学调控，从已有报道的十字花科植物挥发性化合物中选取11种化合物进行测试和引诱剂配方设计，这11种化合物（乙酸苯甲酯、香叶醇、苯甲醛、苯乙醇、丁香酚、苯甲醇、苯乙酮、苯乙醛、水杨醛、肉桂醛、水杨酸甲酯）均能能激发菜粉蝶和其他鳞翅目昆虫触角电位（EAG）或其他生理反应。以正己醇为参照，室内系统测定了菜粉蝶成虫对上述11种化合物的EAG反应，然后采用配方均匀设计方法组配了四组配方，于2009-2011年共4个时期在野外进行了大田诱捕试验。在EAG测试中，菜粉蝶成虫对用作参照的正己醇（一种绿叶气味）反应最强，对所测试的11种化合物均有不同的反应，但均比对正己醇弱，这可能意味着菜粉蝶在长期进化过程中所形成的搜寻寄主植物的行为，首先应当是寻找“绿色植物”，再考虑其他因素。在大田诱捕试验中，配方1、配方2的诱捕量相对较少，各组分对诱捕量的贡献没有显著差异，配方4的诱捕量最大，不同化合物样品的EAG反应值和野外诱捕效力不存在明显的相关性；关键化合物苯乙醛起着主导作用，这一点已在此前的许多报道中证实，但另一种很常见的花香气味2-苯乙醇则效果并不强。在2009年6月10日至7月10日的诱捕实验中首次发现，水杨酸甲酯具有较强的引诱作用。本研究表明，触角电位的生理数据不能代替大田试验，菜粉蝶对十字花科挥发性化合物的行为反应还需考虑挥发性化合物相互作用、化学信息多义及其与信息素相互叠加编码等现象。 |
| 英文摘要: |
| It is a new strategy that larval populations can be suppressed through trapping and killing adults of insect pests. In order to provide effective trapping formulations based on chemical ecological management strategies for control of Pieris rapae populations, eleven plant volatile chemicals from Brassicaceae were selected for the laboratory and field experiments, including Benzyl acetate，Geraniol，Benzaldehyde，Phenylethyl alcohol，Eugenol，Phenyl methanol，Acetophenone，Phenylacetaldehyde，Salicylaldehyde，Cinnamaldehyde and Methyl salicylate. These chemicals were reported being able to trigger electroantennographic (EAG) or other behavioral responses in P. rapae and other lepidopteron insects. The laboratory bioassays on the antennal responses of P. rapae to these volatiles using EAG technology were carried out with hexanol as a reference chemical. A series of field trapping experiments were conducted from 2009 to 2011, using some formulae based on Mixture of Uniform (UM) Design. In the bioassay of EAG, response of the butterfly adults to hexanol (a green leaf volatile) was the highest among volatile substances tested inspiring EAG responses, which may suggest that the P. rapae should first learn to search for “green plants” in the long-term evolution process of host-plant searching behaviors rather than considering other factors. In the field trapping experiments, the formula 1 and formula 2 trapped less P. rapae than other formulae, and the contribution of different components to the trap-catch amounts was similar. The formula 4 trapped the largest amount of P. rapae with various ratios of different compounds. There was no direct correlation between EAG response values of the volatiles and field trapping effects. The compound acetophenone played a key role in field trapping, which was confirmed by previous reports, but another very common floral odor, phenylethyl alcohol, had a weak effect. In the trapping experiment in the period from June 10 to July 10, 2009, the methyl salicylate was found to have strong attractiveness on P. rapae. Our results indicated that the physiological data of EAG cannot replace the field test, and the chemical information interaction, polysemy and pheromone superimposed coding phenomenon should also be considered in experiments on the behavioral responses of P. rapae to Brassicaceae volatiles. |
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