| 徐广增1，刘守柱1*，梁婷婷2，楚成茹1，潘立婷1，李婷1,2015,刚竹叶提取物对斜纹夜蛾酚氧化酶抑制作用研究[J].环境昆虫学报,37(5):1030-1036 |
| 刚竹叶提取物对斜纹夜蛾酚氧化酶抑制作用研究 |
| Inhibition effects of bamboo extracts from Phyllostachys viridisagainst phenoloxidase from Spodoptera litura Fabricius |
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| DOI： |
| 中文关键词: 刚竹 提取物 斜纹夜蛾 酚氧化酶 抑制作用 |
| 英文关键词:Phyllostachys viridis extract Spodoptera litura phenoloxidase inhibition |
| 基金项目:聊城大学博士科研启动基金（31805）；聊城大学“园艺植物病虫害控制技术研究”重点建设项目（13kz0801） |
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| 中文摘要: |
| 采用酶标仪法测定了刚竹叶提取物对斜纹夜蛾酚氧化酶（PO）活性的影响。结果表明：6种不同溶剂（极性大小：去离子水>乙醇>丙酮>乙酸乙酯>氯仿>石油醚）获得的竹叶提取物均对PO表现为抑制作用（三氯甲烷提取物在0-300 mg/L范围内，对PO表现为激活作用），且随提取物浓度的升高，抑制作用有逐渐增强的趋势。丙酮提取物对PO的抑制效果最显著（IC50为550 mg/L），其次为乙酸乙酯提取物和乙醇提取物（IC50分别为575 mg/L和700 mg/L）；丙酮提取物对斜纹夜蛾酚氧化酶的抑制效果属于可逆过程，抑制类型为非竞争性抑制，抑制常数Ki为158.2 mg/L。 |
| 英文摘要: |
| Inhibition effects of bamboo extracts against phenoloxidase (PO) from Spodoptera lituraFabricius was determined with microplate reader. The results showed that: the bamboo leaf extracts obtained from 6 different solvents (polarity: deionized water>ethanol>acetone>ethyl acetate>chloroform>petroleum ether) have inhibitory effects against PO (only chloroform extracts showed the activation effect within the concentration from 0 to 300 mg/L）. Besides, the inhibition was gradually enhanced along with the increase of extracts’concentration. Acetone extracts showed the most obvious inhibitory effect against PO and the IC50 was 550 mg/L. Ethyl acetate and ethanol extracts also have good inhibition effects, and the IC50 were 575 mg/L and 700 mg/L respectively. The inhibition effect of acetone extract against PO was reversible. It was determined that acetone extract is a noncompetitive inhibitor, and the Ki value is 158.2 mg/L. |
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