,2015,一株高效防治甜菜夜蛾的莱氏野村菌的筛选[J].环境昆虫学报,37(4):910-914
一株高效防治甜菜夜蛾的莱氏野村菌的筛选
Screening of a Nomuraea rileyistrain with high insecticidal activity against Spodoptera exigua
  
DOI:
中文关键词:  莱氏野村菌  甜菜夜蛾  3龄幼虫  致病性
英文关键词:Nomuraea rileyi  Spodoptera exigua  3rd instar larvae  pathogenicity
基金项目:江苏省农业科技自主创新资金(CX(12)3059)
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中文摘要:
      从罹病斜纹夜蛾中分离获得一杀虫真菌菌株Nr101,经ITS鉴定该菌为莱氏野村菌Nomuraea rileyi。室内采用浸渍法测定了其5个浓度(2.25×104-2.25×108个孢子/mL)对3龄甜菜夜蛾的毒力。施药后8 d 后,2.25×108、2.25×107、2.25×106、2.25×105、2.25×104个孢子/mL浓度下,甜菜夜蛾3龄幼虫死亡率分别为100%、97.5%、86.67%、57.5%、43.33%。2.25×105、2.25×106、2..25×107、2.25×108 的LT50 分别为7.4165、5.7384、4.7908、4.3292 d。药后8 d LC50为6.2180×104,显示出很好的应用前景。
英文摘要:
      A Nomuraea rileyi strain Nr101 was isolated from dead larvae of Spodoptera litura.The pathogenicity of Nr101 to the 3rd instar larvae ofSpodoptera exigua under five different concentrations from 2.25×104 to 2.25×108 spores/mL was determined. The results indicated that the mortality rate of the 3rd instar larvae caused by N. rileyi Nr101 was 100%, 97.50%, 86.67%, 57.50% and 43.33%, respectively, under the concentration of 2.25×108, 2.25×107, 2.25×106, 2.25×105 and 2.25×104 spores/mL. The LT50 values of the 3rd instar larvae were 7.4165, 5.7384, 4.7908 and 4.3292 d, respectively, under different concentrations (2.25×106, 2.25×107, 2.25×108 spores/mL). At the 8th day, the LC50 of the larvae was 6.2180×104 spores/mL, which demonstrated it is a promising fungal insecticide.
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