| ,2015,防治稻水象甲优效球孢白僵菌 YS03 菌株产孢培养基优化[J].环境昆虫学报,37(4):800-806 |
| 防治稻水象甲优效球孢白僵菌 YS03 菌株产孢培养基优化 |
| Optimization of sporulation medium of Beauveria bassianaYS03 controling rice water weevil Lissorhoptrus oryzophilus |
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| DOI： |
| 中文关键词: 稻水象甲 YS03 球孢白僵菌 培养基 产孢量 响应面优化 |
| 英文关键词:Lissorhoptrus oryzophilus Beauveria bassiana YS03 medium the spore yields response surface methodology (RSM) |
| 基金项目:贵州省农业科技攻关项目（黔科合NY字\[2014\]3015号） |
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| 中文摘要: |
| 采用单因素试验和响应面分析对球孢白僵菌 YS03 菌株进行产孢培养基的配方改良。单因素试验筛选出对产孢量影响较大的3种原料为花生、麦麸和玉米粉，进而根据Box-Behnken的中心组合原理进行三因素三水平的实验设计，最终得出使产孢量最大的配方为花生∶麦麸∶玉米粉=2.6∶3.5∶3.9。经验证，预测值与验证试验平均值接近，且新配方的产孢量是PDA培养基产孢量的1.92倍。 |
| 英文摘要: |
| The formula ofBeauveria bassiana YS03 culture mediums was improved by single factor test and response surface methodology. The important factors influencing the spore yields, which identified by initial screening method of single factor test, were peanut, wheat bran and corn flour. Ingredients were obtained through Box-Benhnken central combination design and RSM. The results showed that the optimum yields conditions of spore are as follows: peanut∶wheat bran∶corn flour= 2.6∶3.5∶3.9. After experimental verification, the maximum predicted value was consistent with mean value and optimal medium was as 1.92 times as that of PDA medium. |
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