| ,2015,棉铃虫 Wnt1 基因启动子活性研究[J].环境昆虫学报,37(4):778-784 |
| 棉铃虫 Wnt1 基因启动子活性研究 |
| Promoter activity analysis of Helicoverpa armigeraWnt1 gene |
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| DOI： |
| 中文关键词: 棉铃虫 染色体步移 启动子活性分析 Wnt1 启动子 |
| 英文关键词:Helicoverpa aimigera genome walking technique promoter activity analysis Wnt1 promoter |
| 基金项目:国家自然科学基金项目（31230066）； 国家重点基础研究发展计划项目（2012CB114101） |
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| 中文摘要: |
| 经典的 Wnt 信号通路是一条与生长发育密切相关的信号通路，经典 Wnt 信号通路在棉铃虫滞育蛹脑中受到抑制。本研究以棉铃虫 Wnt1 基因的转录调控为研究目标，对 Wnt1 启动子的活性进行了分析。首先，运用染色体步移的技术成功克隆了一段长为1566 bp的 Wnt 1启动子。然后，通过PCR扩增不同长度的启动子片段，构建到报告质粒，转染棉铃虫HzAm1细胞并进行启动子活性分析，结果表明-1077~-1120以及-1120~-1140这两个区域对 Wnt1 基因的转录起着明显的激活作用。最后对这两个区域潜在的转录因子结合位点进行了预测。本研究从根本上了解棉铃虫滞育蛹脑中经典Wnt 信号通路的调节机制打下了基础。 |
| 英文摘要: |
| Classical Wnt signaling pathway, a pathway closely realted to development, is inhibited in the Helicoverpa armigera pupal brain during diapause. This study aims to study the transcriptional regulation mechanism of Wnt1 gene by analyzing Wnt1promoter activity. Firstly, a 1566 bp fragment of the promoter of Wnt1 gene was cloned using genome walking technique. Then, series of constructs that contained different lengths of possible Wnt1promoters were fused to luciferase reporter gene, and the constructed plasmids were transfected into HzAm1 cells. The result indicates that sequences between-1077~-1120 and -1120~-1140 can significantly enhance the transcriptional activity of Wnt1promoter. And finally, the potential sequences for regulatory elements in the promoter were analyzed. This study lays the foundation for the further regulating mechanism research about classical Wnt signaling pathway in the Helicoverpa armigerapupal brain during diapause. |
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