| ,2015,沟眶象体内感染的 Wolbachia 的 wsp 基因克隆与序列分析[J].环境昆虫学报,37(4):752-758 |
| 沟眶象体内感染的 Wolbachia 的 wsp 基因克隆与序列分析 |
| Cloning and sequence analysis of the wsp gene of Wolbachia in Eucryptorrhynchus chinensis |
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| DOI： |
| 中文关键词: 沟眶象 wsp 克隆 系统发育 Wolbachia |
| 英文关键词:Eucryptorrhynchus chinensis wsp clone sequence phylogeny Wolbachia |
| 基金项目:林业公益性行业科研专项（201304412）；十二五科技支撑计划课题(2012BAD19B07 ) |
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| 中文摘要: |
| Wolbachia 是广泛分布于昆虫细胞内的一类共生菌，为明确 Wolbachia 在沟眶象 Eucryptorrhynchus chinensis (Olivier) 体内的感染情况和分类地位，采用 Wolbachia 的 wsp 基因片段对沟眶象5个地理种群（宁夏灵武、中宁、中卫、安徽淮北、山东聊城）共100个样本，进行分子检测。经过克隆和测序得到 wsp 基因片段593 bp（GenBank number：KP713759），明确了沟眶象体内存在 Wolbachia 的感染，并且其在不同地理种群内的感染率均为100%；通过 wsp 通用引物检测发现沟眶象体内感染了1种 Wolbachia，属于A群Dro亚群。 |
| 英文摘要: |
| Bacteria of the genus Wolbachiaare widespread in arthropods. The infection and evolution position of Wolbachiain Eucryptorrhynchus chinensis ( Coleoptera: Curculionidae ) was identified. By using specific primer for wsp gene of Wolbachia and PCR method, we investigated the Wolbachiainfection of populations of E. chinensis in China. These populations were from Ningxia Lingwu, Zhongning, Zhongwei, Anhui Huaibei, Shandong Liaocheng. After PCR and clone sequence, a 593 bp region of the Wolbachiawsp gene ( GenBank number: KP713759 ) was amplified. All populations were infected withWolbachia. Phylogenetic analysis showed that Wolbachiain E. chinensis belongs to the Dro subgroup of the A group. |
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