| ,2014,龄期、饲养条件及生测方法对韭菜迟眼蕈蚊对药剂敏感性的影响[J].环境昆虫学报,(5):730-743 |
| 龄期、饲养条件及生测方法对韭菜迟眼蕈蚊对药剂敏感性的影响 |
| Effect of larval instars, rearing conditions and bioassay methods to the insecticide susceptibility of Bradysia odoriphaga |
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| DOI： |
| 中文关键词: 韭菜迟眼蕈蚊 龄期 饲养条件 药剂敏感性 生测方法 |
| 英文关键词:Bradysia odoriphaga larval instars rearing conditions bioassay methods insecticide susceptibility |
| 基金项目:公益性行业(农业)科研专项经费资助（201303027） |
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| 中文摘要: |
| 为建立针对韭蛆的简便、可靠的生测方法，采用胃毒触杀联合毒力法、人工饲料混药法和浸虫法分别测定毒死蜱、噻虫胺对韭蛆不同龄期幼虫的毒力，以明确龄期、温度和饲料等饲养条件及生测方法对韭菜迟眼蕈蚊对药剂敏感性测定结果的影响。三种方法测得2龄幼虫对毒死蜱的敏感性分别为4龄幼虫的11.9倍、7.4倍、3.3倍；4种饲料饲养的试虫，毒死蜱对取食韭菜、人工饲料、大葱的LC50分别为圆葱的1.84倍、1.47倍、2.31倍；在15~30℃范围内，随着试虫的饲养温度的升高，毒死蜱对韭蛆4龄幼虫的LC50不断增大；三种方法测得毒死蜱对4龄幼虫的LC50分别为67.199 mg/L、71.123 mg/L、88.120 mg/L，噻虫胺对4龄幼虫的LC50依次为3.584 mg/L、2.255 mg/L、4.329mg/L，并且胃毒触杀联合毒力法和人工饲料混药法的毒力曲线拟合度要好于浸虫法。试验表明：试虫龄期、饲养温度、饲养饲料等因素对韭菜迟眼蕈蚊对药剂敏感性存在明显影响，采用胃毒触杀联合毒力法和人工饲料混药法测定的结果比浸虫法准确可靠。 |
| 英文摘要: |
| In order to establish a simple and reliable bioassay method to Bradysia odoriphaga, this experiment evaluated the effects of test factors to the toxicity, including different larval instars, the rearing temperatures and the different rearing diets, and we determined the effects to bioassay results of the insecticide susceptibility of B. odoriphaga to Chlorpyrifos and Clothianidin by stomach-contact toxicity method, artificial diet mixed with agent method and dip method. The results showed that the sensibility of second instar larvae to Chlorpyrifos were 11.9-, 7.4- and 3.3-folds respectively compared with the forth instar larvae measured by the three bioassay methods. For the test insects fed with these four different diets, LC50 of the insects fed on the chinese chive, artificial diet and welsh onion to Chlorpyrifos were 1.84-, 1.47- and 2.31-folds compared with the insects fed on onion. In the range from15℃ to 30℃, LC50 of forth larval instars of B. odoriphaga to Chlorpyrifos increased as the rearing temperature rose. Tested by these three methods, LC50 of forth larval instars to Chlorpyrifos were 67.199 mg/L, 71.123 mg/L, 88.120 mg/L respectively while LC50 of forth larval instars to Clothianidin was 3.584 mg/L, 2.255 mg/L, 4.329mg/L respectively and the fitting degrees of the toxicity curve of stomach-contact toxicity method and artificial diet mixed drugs method were superior to dip method. The study showed that the test insect instars, the rearing temperatures, the different rearing diets had a significant influence on the insecticide susceptibility of B. odoriphaga. The results tested by the stomach-contact toxicity method and artificial diet mixed drugs method were more reliable and accurate than the dip method. |
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