| ,2014,内蒙古11种主要草原蝗虫16S rDNA序列分析[J].环境昆虫学报,(4):525-530 |
| 内蒙古11种主要草原蝗虫16S rDNA序列分析 |
| Sequence analysis of 16S rDNA of 11 main grasshopper species in Inner Mongolia grassland |
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| DOI： |
| 中文关键词: 蝗虫；16S rDNA 序列分析；分子系统发育 |
| 英文关键词:grasshopper 16S rDNA sequence analysis molecular phylogeny |
| 基金项目:国家公益性行业（农业）科研专项（201003079） |
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| 中文摘要: |
| 测定了内蒙古11种主要草原蝗虫的线粒体基因组16S rDNA序列，并构建了其分子系统树。在获得的505 pb序列中，G+C占302%，A+T占698%，表现出A/T偏向性。在505 bp个碱基中检测到156个多态性位点，占碱基总数的309%。156个多态性位点中包括50个单变异多态性位点（占总位点数的321%）和106个简约信息位点（占总位点数的679%）。分别采用NJ、ME、MP和UPGMA聚类方法构建系统发生树。结果表明，4科的17种蝗虫共聚为6支，其中槌角蝗科、斑腿蝗科与网翅蝗科3个科的蝗虫先聚在一起，再与斑翅蝗科相聚。4种聚类方法中UPGMA聚类法更为符合传统的以形态学为基础的分类体系。 |
| 英文摘要: |
| The 16S rDNA sequences of 11 important grasshopper species distributed in Inner Mongolia grassland were analyzed In the 505 bp fragment of mitochondial 16S rDNA, G+C was 302% and A+T was 698%, showing a tendency to A/T 156 polymorphic loci were detected in 505 bp base, accounting for 309% of the total number of bases, including 50 single variation polymorphic sites and 106 parsimony informative sites The molecular phylogenetic trees were constructed by NJ, ME, MP and UPGMA, respectively The results showed that 17 grasshopper species from 4 families were clustered into 6 groups Gomphoceridae, Catantopidae and Arcypteridae were firstly clustered and then clustered with Oedipodidae Among four clustering methods, UPGMA result was consilient to the classification scheme of Acrioidea based on morphology. |
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