| ,2014,脂氧合酶OsRCI-1正调控水稻对二化螟的抗性[J].环境昆虫学报,(4):507-515 |
| 脂氧合酶OsRCI-1正调控水稻对二化螟的抗性 |
| Rice lipoxygenase gene OsRCI-1 mediated pathway positively regulates resistance to Rice Striped Stem Borer |
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| DOI： |
| 中文关键词: 水稻 OsRCI-1 脂氧合酶 茉莉酸 虫害诱导防御 |
| 英文关键词:OsRCI-1 lipoxygenase jasmonic acid herbivore-induced defence |
| 基金项目:国家自然科学基金(31101451)；浙江省自然科学基金(Y3100384)；浙江农林大学“青年拨尖人才”和校发展基金(2034070001、2034020061)；浙江农林大学研究生科研创新基础项目(3122013240262)；2013年浙江省大学生科技创新活动计划项目（2013R412037） |
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| 中文摘要: |
| 本文克隆了虫害诱导的水稻脂氧合酶基因OsRCI-1全编码序列， 实时定量QRT-PCR 检测结果表明二化螟Chilo suppressalia Walker取食能快速且持续地诱导OsRCI-1基因的表达。利用农杆菌介导的水稻遗传转化获得了两株OsRCI-1基因的RNAi沉默突变体，突变体株系ir-rci-1和ir-rci-2中目的基因OsRCI-1的表达被明显的抑制，其表达水平分别仅为对照(秀水11)的4773%和3233%。生测结果表明OsRCI-1基因沉默导致水稻对二化螟抗性显著地降低，取食RNAi突变体株系的二化螟体重分别是取食秀水11的158倍和215倍。因此，水稻OsRCI-1基因正调控对二化螟的抗性，该基因介导的防御途径可能在水稻虫害诱导防御反应中起重要的作用。 |
| 英文摘要: |
| We isolated an herbivore-induced rice lipoxygenase gene OsRCI-1 with full coding sequence (CDS) and its expression levels quickly and constantly increased after rice Striped stem borer (SSB), Chilo suppressalia Walker treatment Using Agrobacterium-mediated transformation technology, we develop two mutant lines of OsRCI-1 (ir-rci-1 and ir-rci-2)，in which the expression levels of OsRCI-1 were inhibited significantly, the transcript level of OsRCI-1 in ir-rci-1 and -2 was only 4773% and 3233% of level of XS11 Bioassay showed that resistance toward SSB was greatly impaired in mutant rice plants ir-rci lines compared to wild type rice (WT). The mass of SSB that fed on ir-rci lines was 158 and 215 times comparing with WT plants, respectively The results indicated that OsRCI-1 gene positively regulated resistance of rice toward rice SSB, which may play an important role in herbivore-indcued defence in rice. |
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