| ,2014,桔小实蝇Rab家族基因鉴定和表达模式研究[J].环境昆虫学报,(1):71-77 |
| 桔小实蝇Rab家族基因鉴定和表达模式研究 |
| Identification and gene expression pattern analysis of Rab family genes in Bactrocera dorsalis(Diptera: Tephritidae) |
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| DOI： |
| 中文关键词: 桔小实蝇 Rab 表达模式 |
| 英文关键词:Bactrocera dorsalis Rab expression pattern |
| 基金项目:公益性行业（农业）科研专项经费项目（200903047）;国家自然科学基金项目（31201516）；国家现代农业（柑橘）产业技术体系（ CARS-27） |
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| 中文摘要: |
| 桔小实蝇Bactrocera dorsalis（Hendel）是国内重要的果蔬害虫，能取食多种水果和蔬菜，给果蔬业造成严重的经济损失。Rab蛋白是一类小分子GTP 结合蛋白，在囊泡运输和信号传递中发挥了重要作用。本研究从桔小实蝇转录组文库中鉴定了7个Rab基因家族作为研究对象，利用实时荧光定量PCR等分子生物学技术研究了其表达模式。研究结果不仅有助于提高我们对桔小实蝇发育分子机制的理解，而且为防治桔小实蝇提供了新思路和新途径。 |
| 英文摘要: |
| Bactrocera dorsalis (Hendel) is a destructive insect species that can cause severe economic losses to fruit and vegetable industry. Rab protein is a class of small GTP binding protein which is important for vesicular trafficking and signal transduction.Seven Rab family genes of B. dorsalis transcriptome library was identified as the object of study in this research. Molecular biological techniques like real-time fluorescence quantification PCR were employed to study the genes expression profiles. The results has not only enhanced our understanding of the B. dorsalis development mechanism, but also provided new ways and possibilities to prevent and control the insect. |
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