《环境昆虫学报》

昆虫体内的解毒酶活性是反映其抗药性水平的主要生理指标，本文比较了桔小实蝇Bactrocera dorsalis的几种解毒酶活性和抗药性水平关系。测定了9个地理品系和相对敏感品系的桔小实蝇成虫的三种解毒酶，即多功能氧化酶(Mixed function oxidase, MFO)、羧酸酯酶（Carboxylesterase, CarE）和谷胱甘肽Ｓ-转移酶(Glutathione S-transferase, GST)的活性,利用药膜法测定其对敌百虫(Trichlorphon)、高效氯氰菊酯(β-cypermethrin)和阿维菌素(Avermectin)的抗性水平，比较了各地理品系的桔小实蝇这些酶活性和对不同杀虫剂的抗性水平的关系，并作通径分析。结果表明：广东广州地区桔小实蝇体内的MFO-O-脱甲基活性最高，为相对敏感品系酶活性的1.4782倍；而广东茂名地区品系酶活性最低，只有0.8649倍。广东惠州地区桔小实蝇体内CarE活性最高，为相对敏感品系酶活性1.8147倍；而广西南宁地区的桔小实蝇体内酶活性最低，为敏感品系的0.9636倍。广东茂名地区桔小实蝇GST活性最高，是相对敏感品系2.2557倍；而广东广州地区桔小实蝇GST活性最低，只有相对敏感品系的1.1622倍。抗性水平表明：各地理品系对敌百虫的抗性水平为相对敏感品系的2.1552倍至100.2271倍之间，对高效氯氰菊酯的抗性水平在1.0065到26.0026倍之间，对阿维菌素的抗性水平在2.3353倍至29.0688倍之间。相关性分析表明：桔小实蝇体内的GST活性和对敌百虫抗性水平的相关系数为0.41，存在显著性正相关；CarE活性与桔小实蝇对高效氯氰菊酯抗性水平存在极显著正相关，相关系数为0.50。通径分析结果表明：GST活性对敌百虫的抗药性水平的直接通径系数为0.4414，对敌百虫的抗性上升起到正向作用；MFO-O-脱甲基活性、CarE活性和GST活性对高效氯氰菊酯抗性水平的直接通径系数分别为0.3311，0.4946和0.1775，均起到正向作用；GST活性与阿维菌素的抗性水平的直接通径系数很小，为0.0668。结果显示了桔小实蝇的解毒酶与抗药性水平关系密切，在抗性发展中起到了促进作用。

英文摘要:

Detoxification enzymes activities in insect are main physiological traits that indicated their resistant levels. The relationship between detoxification enzymes activities and resistance levels of oriental fruit fly, Bactrocera dorsalis (Hendel) were compared in the article. The specific activities of three detoxification enzymes such as Mixed function oxidase (MFO), Carboxylesterase (CarE) and Glutathione S-transferase (GST) in the 9 different populations and relative sensitive strains of oriental fruit fly, Bactrocera dorsalis, were determined in this study. And their insecticide resistance to trichlorphon, β-cypermethrin and avermectin were measured by film residue method. Furthermore, the relationship and path analysis between enzyme activities and resistance levels to the three insecticides were analyzed. The results of enzyme activity assays showed that the activity of MFO-O-demethylase of oriental fruit flies in Guangzhou population was higher than the others, but its value in Maoming strain was the lowest, and they were 1.4782 and 0.8649 times to sensitive strain, respectively The CarE activity in Huizhou population was the highest as 1.8147 times to sensitive strains, and its in Nanning colony was the lowest, only as 09636 times to sensitive strain The activities of GST in Maoming population were the highest as 2.2557 times to sensitive strain, and its activities in Guangzhou population were the lowest as 1.1622 times to sensitive strains The resistance levels of geographical populations showed that resistant multiple to trichlorphon were from 2.1552 to 100.2271, and from 1.0065 to 26.0026, and from 2.3353 to 29.0688 to β-cypermethrin to avermectin The correlation analysis results indicated that correlation coefficient between GST activity and resistance level to trichlorphon was 0.41, as significantly positively correlated And CarE activity and resistance of β-cypermethrin was also significantly positively correlated and its correlation coefficient was 0.50 The results of path analysis showed that direct path coefficients between GST activity for resistance to trichlorphon were 04414, indicated that positive effects of GST to oriental fruit fly resistance development to trichlorphon And direct path coefficients of MFO-O-demethylase, CarE and GST activities for β-cypermethrin resistance were 0.3311, 0.4946 and 0.1775, and respectively, and suggested that their positive effects on β-cypermethrin resistance in oriental fruit fly However, there was only one direct path coefficient that was GST activity for avermectin resistance was low at 0.0668 All results enlightened the detoxification enzymes played crucial roles on insecticide resistance of Bactrocera dorsalis

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