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| 联苯菊酯对西方蜜蜂生存、生理和肠道菌群的影响 |
| Effects of bifenthrin on the survival, physiology and gut microbiota in honeybees (Apis mellifera L.) |
| 投稿时间：2024-07-11 修订日期：2024-09-11 |
| DOI： |
| 中文关键词: 西方蜜蜂 联苯菊酯 生存 基因表达 肠道菌群 |
| 英文关键词:Apis mellifera L. Bifenthrin Survival Genes expression Gut microbiota |
| 基金项目:国家自然科学(32102604)；国家蜂产业技术体系建设专项资金项目(CARS-45-KXJ10) # |
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| 中文摘要: |
| 联苯菊酯广泛应用于蜜源植物害虫防治中,可能会对蜜蜂健康造成危害。本文主要探究了亚致死浓度联苯菊酯对西方蜜蜂生存、取食、免疫解毒基因表达及肠道菌群的影响。利用5 mg/L和0.5 mg/L联苯菊酯对西方蜜蜂长期饲喂 7 d,统计各组蜜蜂的生存率和取食量；利用qPCR技术检测蜜蜂中肠免疫和解毒基因表达变化；采用16S rDNA测序技术检测蜜蜂肠道菌群丰度、结构变化。结果显示：(1)5 mg/L联苯菊酯能够显著降低蜜蜂的生存率和花粉取食量(P < 0.05),而对糖水取食量没有显著影响；(2)5 mg/L联苯菊酯显著抑制CYP6AS3和CYP6AS10基因的表达(P < 0.05),而诱导Abaecin、Apidaecin、Defensin、Hymenoptaecin基因上调表达(P < 0.05)；0.5 mg/L联苯菊酯显著抑制Lysozyme、CYP6AS10基因的表达(P < 0.05),而诱导Apidaecin、Defensin基因上调表达(P < 0.05)；(3)联苯菊酯处理能够诱导蜜蜂肠道菌群的多样性发生变化,并影响特定肠道菌的丰度。结果表明联苯菊酯曝露不仅损害西方蜜蜂生存和取食,并对其免疫解毒系统以及肠道稳态造成影响。 |
| 英文摘要: |
| Bifenthrin is widely used for pest control in nectariferous crop, which may harm honeybees (Apis mellifera L.) health. This study aims to investigate effects of sublethal concentrations of bifenthrin on survival, feeding, immune and detoxification related genes expression and the gut microbiota in honeybees. The honeybees were fed with 5 mg/L and 0.5 mg/L bifenthrin for 7 d, and the survival and food consumption in each group were calculated. Changes in the expression of immune and detoxification related genes in honeybee midguts were detected by qPCR, and the abundance and structural of gut microbiota was analyzed by 16S rDNA sequencing technology. The results showed that: (1) 5 mg/L bifenthrin significantly reduced the survival and pollen consumption (P < 0.05), but had no significant effect on syrup consumption in honeybees; (2) 5 mg/L bifenthrin significantly inhibited CYP6AS3 and CYP6AS10 expression (P < 0.05), while up-regulated the expression of Abaecin, Apidaecin, Defensin and Hymenoptaecin (P < 0.05); 0.5mg /L bifenthrin significantly inhibited Lysozyme and CYP6AS10 expression (P < 0.05), while up-regulated the expression of Apidaecin and Defensin (P < 0.05); (3) Bifenthrin treatment can induce changes in Alpha and Beta diversity, and affect the abundance of specific bacteria. This study indicates that bifenthrin exposure not only damages the survival and food consumption, but also influences the immune and detoxification systems and intestinal homeostasis in honeybees. |
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