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| 莱氏绿僵菌MDXZ200803和SLSY200714对草地贪夜蛾幼虫致病性差异的研究 |
| The pathogenicity difference of Metarhizium rileyi MDXZ200803 and SLSY200714 to the larvae of Spodoptera frugiperda |
| 投稿时间：2024-01-06 修订日期：2024-09-22 |
| DOI： |
| 中文关键词: 莱氏绿僵菌 草地贪夜蛾幼虫 毒力 保护酶 解毒酶 |
| 英文关键词:Metarhizium rileyi spodoptera frugiperda larvae virulence protective enzymes detoxifying enzymes |
| 基金项目:云南省自然科学基金面上项目(202201AT070976)；云南省教育厅科研(2023Y0902) |
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| 中文摘要: |
| 为了研究莱氏绿僵菌Metarhizium rileyi对草地贪夜蛾Spodoptera frugiperda幼虫的致病机制，本研究在田间草地贪夜蛾幼虫罹病虫体上分离鉴定了两株莱氏绿僵菌MDXZ200803和SLSY200714。采用浸渍法分别测定5个孢子浓度（1×104~1×108 个/mL）的莱氏绿僵菌对3龄和5龄草地贪夜蛾幼虫的毒力以及侵染后3龄草地贪夜蛾幼虫保护酶（POD、CAT和SOD）和解毒酶（GST和CarE）的变化。研究结果表明，莱氏绿僵菌菌株MDXZ200803侵染草地贪夜蛾3龄幼虫8 d后，1×107和1×108 个/mL孢子浓度的累计校正死亡率均达到100%，对5龄幼虫的累计死亡率都未达到100%。莱氏绿僵菌菌株SLSY200714侵染草地贪夜蛾3龄幼虫8 d后，只有1×108 个/mL孢子浓度的累计死亡率达到100%，对5龄幼虫的累计死亡率则均未达到100%。莱氏绿僵菌1×108 个/mL孢子悬浮液处理草地贪夜蛾3龄幼虫36、48、60和72 h后，CAT、SOD和CarE的酶活性在处理36 h时最高，随后逐渐下降，而POD和GST酶活性在48 h时最高，之后下降。本研究从宿主免疫酶活性变化角度出发，为揭示莱氏绿僵菌的致病机制提供了一定的理论依据，同时为草地贪夜蛾的绿色防控奠定了基础。 |
| 英文摘要: |
| In order to study the pathogenic mechanism of Metarhizium rileyi on Spodoptera frugiperda larvae, two strains of M. rileyi MDXZ200803 and SLSY200714 were isolated and identified from the diseased larvae of S. frugiperda in the field. The virulence of M. rileyi with five spore concentrations (1×104~1×108 conidia/mL) to the 3rd and 5th instar larvae of S.frugiperda and the changes of protective enzymes ( POD, CAT and SOD ) and detoxification enzymes ( GST and CarE ) in 3rd instar larvae infected by two strains of M. rileyi were determined by the impregnation method. The results showed that the cumulative corrected mortality of the 3rd instar larvae of S. frugiperda was 100% at the concentration of 1×107 and 1×108 conidia / mL after 8 days of infection with the strain MDXZ200803, and the cumulative corrected mortality of the 5th instar larvae did not reach 100%. M. rileyi SLSY200714 infected the 3rd instar larvae of S. frugiperda for 8 days, only the cumulative corrected mortality at the concentration of 1×108 conidia / mL reached 100%, and the cumulative corrected mortality for the 5th instar larvae did not reach 100%. After 3rd instar larvae of S. frugiperda were treated with 1×108 conidia / mL for 36, 48, 60 and 72 h, the activities of CAT, SOD and CarE were the highest at 36 h and then decreased gradually, while the activities of POD and GST were the highest at 48 h and then decreased. This study provides a theoretical basis for revealing the pathogenic mechanism of M. rileyi from the perspective of host immune enzyme activity changes, and provides ideas for the biological control against S. frugiperda. |
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