| 夏国益，毛根林，董建豪，徐玉辉，路伟,2026,臭椿酮抑制斜纹夜蛾幼虫生长发育的差异代谢物分析[J].环境昆虫学报,(3):908-916 |
| 臭椿酮抑制斜纹夜蛾幼虫生长发育的差异代谢物分析 |
| Metabolomic analysis of ailanthone-induced alterations in the growth and development of Spodoptera litura larvae |
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| DOI： |
| 中文关键词: 臭椿酮 斜纹夜蛾 液质联用 生长发育 代谢组学 |
| 英文关键词:Ailanthone Spodoptera litura liquid chromatography tandem mass spectrometry growth and development metabolomics |
| 基金项目:新疆维吾尔自治区重点研发专项（2022B02033-1）；新疆维吾尔自治区“天山英才”三农骨干人才计划（2023SNGGGCC032）；新疆棉花产业技术体系（XJARS-03）；国家自然科学基金青年基金（32102225） |
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| 中文摘要: |
| 【目的】为明确臭椿酮对斜纹夜蛾Spodoptera litura代谢组的影响，【方法】本研究通过液相色谱质谱联用技术（LC-MS/MS）对臭椿酮处理的斜纹夜蛾3龄幼虫差异代谢物进行检测，并对差异代谢物进行注释、筛选、分类和代谢通路分析。【结果】结果表明，通过非靶向代谢组学技术获得了419个差异代谢物，有157个代谢物上调，262个代谢物下调，主要集中在脂质和类脂分子、有机酸及其衍生物、有机杂环化合物等12类。其中乙酰-L-肉碱、硬脂酰肉碱和葡萄糖酸下调较显著，与对照组相比分别下调了0.38倍、0.47倍和0.51倍；N-亚麻酰基谷氨酰胺、N-棕榈酰谷氨酰胺和谷氨酰丙氨酸分别上调了8.19倍、7.57倍和2.18倍。KEGG通路富集结果显示差异代谢物在嘌呤代谢、丙酮酸代谢、TCA循环等12条代谢通路显著富集。【结论】臭椿酮可引起斜纹夜蛾代谢产物发生明显变化，进一步影响斜纹夜蛾体内氨基酸代谢、能量代谢、嘌呤嘧啶代谢和脂肪酸代谢途径，从而抑制斜纹夜蛾的生长发育。本研究解析了臭椿酮抑制斜纹夜蛾生长发育的代谢调控机制，为该化合物后续开发成植物源农药提供理论依据。 |
| 英文摘要: |
| 【Aim】 This study aims to clarify the effect of ailanthone on the metabolome of Spodoptera litura.【Methods】 Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to detect the differential metabolites of the 3rd instar larvae of Spodoptera litura treated with ailanthone, and the differential metabolites were annotated, screened, classified and analyzed by metabolic pathways. 【Results】The results showed that 419 differential metabolites were identified by non targeted metabolomics techniques, with 157 metabolites upregulated and 262 metabolites downregulated, mainly concentrated in 12 categories such as lipids and lipid-like molecules, organic acid and derivatives, and organoheterocyclic compounds. Among them, the levels of acetyl-L-carnitine, stearoylcarnitine, and gluconic acid were significantly downregulated, reduced to 0.38-, 0.47-, and 0.51-fold of those in the control group, respectively. The levels of N-Linolenoyl glutamine, N-Palmitoleyl glutamine, and Glutamylalanine were upregulated by 8.19-, 7.57-, and 2.18-fold, respectively. The KEGG pathway enrichment results showed that differential metabolites were significantly enriched in 12 metabolic pathways, including purine metabolism, pyruvate metabolism, and TCA cycle.【Conclusion】 Ailanthone induced significant alterations in the metabolites of S. litura, which further affects the amino acid metabolism, energy metabolism, purine pyrimidine metabolism and fatty acid metabolism pathways in S. litura, thereby inhibiting the growth and development of S. litura. This study analyzed the metabolic regulation mechanism of Ailanthone in inhibiting the growth and development of S. litura, providing a theoretical basis for the subsequent development of this compound as a plant-based pesticide. |
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