| 俞显浩，张茜瑜，张雪，林哲，邹振，张俊杰,2026,基于转录组测序探究中红侧沟茧蜂滞育过程中的糖脂代谢[J].环境昆虫学报,(3):890-900 |
| 基于转录组测序探究中红侧沟茧蜂滞育过程中的糖脂代谢 |
| Investigating the glucolipid metabolism of diapause in Microplitis mediator based on transcriptome sequencing |
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| DOI： |
| 中文关键词: 中红侧沟茧蜂 滞育 转录组测序 糖代谢 脂代谢 |
| 英文关键词:Microplitis mediator diapause transcriptome sequencing carbohydrate metabolism lipid metabolism |
| 基金项目:国家青年科学基金项目（32102291） |
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| 中文摘要: |
| 【目的】通过分析中红侧沟茧蜂Microplitis mediator正常发育阶段的蛹和滞育准备阶段的蛹的转录组数据，本研究初步筛选出参与滞育期间能量代谢的相关基因并研究。【方法】利用Illumina HisSeq 2000平台对中红侧沟茧蜂正常发育阶段和滞育准备阶段的蛹进行转录组测序分析，明确了中红侧沟茧蜂正常发育（ND）蛹与滞育准备阶段（DP）蛹之间的转录组数据差异，筛选出参与糖代谢、脂代谢的关键基因，并挑选出其中的10个基因进行RT-qPCR验证。【结果】ND vs DP富集到139 437个差异表达基因，主要富集在参与糖酵解/糖异生、淀粉和蔗糖代谢、脂肪酸降解以及半乳糖代谢等信号通路上。ND vs DP 最富集的GO条目为GO:0005488，被注释为结合功能。共有 7 436 个基因在此条目下富集。根据富集结果筛选出了10个参与糖代谢和脂代谢相关基因，其中参与海藻糖分解的TRE、参与糖原合成的GS和参与TCA的IDH在滞育准备阶段的蛹内的表达量显著高于正常发育阶段的蛹，参与海藻糖合成的TPS和参与TCA循环的FH在滞育准备阶段蛹内的表达量低于正常发育的蛹的表达量；参与脂肪酸代谢与合成的ACOT、ADH、GPAT、FAR和MAGL的表达量在滞育准备阶段的蛹内均高于正常发育的蛹。【结论】本研究根据转录组的结果，分析筛并筛选出10个滞育期间参与糖代谢及脂代谢的相关基因，为后续的研究提供了理论依据。 |
| 英文摘要: |
| 【Aim】Through transcriptomic analysis of Microplitis mediator pupae during the normal developmental stage (ND) and diapause preparation stage (DP), we preliminarily identified and investigated key genes involved in energy metabolism during diapause.【Methods】 Utilizing the Illumina HiSeq 2000 platform, transcriptomic sequencing and analysis were performed on ND and DP pupae. Differences in transcriptomic data between the two stages were identified, key genes involved in carbohydrate and lipid metabolism were screened, and 10 of these genes were selected for RT-qPCR validation.【Results】 A total of 139 437 differentially expressed genes (DEGs) were identified from the ND vs DP comparison. These were primarily enriched in signaling pathways such as glycolysis/gluconeogenesis, starch and sucrose metabolism, fatty acid degradation, and galactose metabolism. The most significantly enriched Gene Ontology (GO) term was GO:0005488, annotated as "binding," which encompassed 7 436 genes. Based on the enrichment results, 10 candidate genes associated with carbohydrate and lipid metabolism were selected. Among these, the expression levels of TRE (involved in trehalose degradation), GS (involved in glycogen synthesis), and IDH (involved in the tricarboxylic acid (TCA) cycle) were significantly higher in DP pupae compared to ND pupae. Conversely, the expression levels of TPS (involved in trehalose synthesis) and FH (involved in the TCA cycle) were lower in DP pupae. Additionally, genes involved in fatty acid metabolism and synthesis, including ACOT, ADH, GPAT, FAR, and MAGL, exhibited higher expression levels in DP pupae than in ND pupae.【Conclusion】 Based on the transcriptome results, this study analyzed and screened 10 key genes involved in carbohydrate and lipid metabolism during diapause, providing theoretical insights for subsequent research in this field. |
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