| 张辉红，徐颢源，郭秀霞，程鹏，王海防，刘丽娟，张崇星,2026,实验室饲养的野外F1代淡色库蚊体表与体内菌群多样性和组成分析[J].环境昆虫学报,(3):864-872 |
| 实验室饲养的野外F1代淡色库蚊体表与体内菌群多样性和组成分析 |
| Diversity and composition analysis of cuticle surface and internal bacterial communities of laboratory-reared field F1 Culex pipiens pallens |
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| DOI： |
| 中文关键词: 淡色库蚊 16S rRNA 高通量测序 多样性 细菌群落 |
| 英文关键词:Culex pipiens pallens 16S rRNA high-throughput sequencing diversity bacterial community |
| 基金项目:山东省自然科学基金项目（ZR2021MH122）；山东省医药卫生科技项目（202412070101） |
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| 中文摘要: |
| 【目的】了解实验室品系（SS）和实验室饲养的野外F1代淡色库蚊Culex pipiens pallens体表与体内菌群多样性和组成差异，可为进一步探究实验室定殖对野外蚊虫菌群影响提供参考。【方法】2023年10月于山东省济宁市唐口镇（TK）和微山湖（WSH）采集淡色库蚊幼虫，转至实验室饲养至F1代。采用16S rRNA测序技术，对在相同条件下饲养的实验室品系和野外F1代淡色库蚊幼虫体内外菌群多样性和组成进行分析。【结果】结果显示：淡色库蚊幼虫体表及体内菌群的优势门主要为变形菌门Proteobacteria、放线菌门Actinobacteriota和厚壁菌门Firmicutes等。α多样性分析显示SS、TK和WSH三组幼虫的体表菌群多样性无统计学差异，而TK组体内菌群的多样性高于SS组。β多样性分析表明，SS组与WSH组体表菌群组成存在显著性差异（P=0.024），且野外F1代幼虫体内菌群与实验室品系均存在显著性差异（TK：P=0.012，WSH：P=0.022）。线性判别分析（Linear discriminant analysis Effect Size，LEfSe）结果显示，三组淡色库蚊幼虫体表存在显著差异的菌群隶属于1门3纲4目5科6属，而体内差异菌群隶属于1门1纲4目5科7属。【结论】综上，在正常实验室饲养条件下，野外淡色库蚊的原生菌群可部分传递至其实验室饲养的F1代幼虫。 |
| 英文摘要: |
| 【Aim】This study investigated the diversity and composition of cuticle surface and internal bacterial communities in larvae of a laboratory strain (SS) versus laboratory-reared F1 larvae derived from wild Culex pipiens pallens populations.【Methods】The findings provided insights into how laboratory colonization influence the bacterial communities of wild mosquitoes. Larvae of Cx. pipiens pallens were collected from Tangkou Town (TK) and Weishan Lake (WSH) in Jining City, Shandong Province, in October 2023, and were subsequently reared in the laboratory to produce the F1 progeny. 16S rRNA sequencing was used to analyse the diversity and composition of the cuticle surface and internal bacterial communities in laboratory strain and laboratory-reared field F1 larvae maintained under identical conditions.【Methods】The results showed that the dominant bacterial phyla of the larval cuticle surfaces and internal bacterial communities were Proteobacteria, Actinobacteriota and Firmicutes. Alpha diversity analyses indicated no statistically significant differences among the cuticle surface bacterial communities of the SS, TK and WSH groups, whereas the internal bacterial diversity in the TK group was significantly higher than that in the SS group. Beta diversity analysis showed significant differences in the cuticle surface bacterial community composition between the SS and WSH groups (P=0.024), and significant differences were also observed in the internal bacterial communities between the laboratory strain and the field-derived F1 larvae (TK: P=0.012，WSH: P=0.022). Linear discriminant analysis Effect Size (LEfSe) demonstrated that the significantly different bacterial taxa on the larval cuticle surfaces among the three groups belonged to 1 phylum, 3 classes, 4 orders, 5 families, and 6 genera. In contrast, the significantly different bacterial taxa in the internal bacterial communities of larvae belonged to 1 phylum, 1 class, 4 orders, 5 families, and 7 genera.【Conclusion】In summary, under standard laboratory rearing conditions, the native bacterial communities from field populations of Cx. pipiens pallens were partially transmitted to their laboratory-reared F1 offspring larvae. |
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