| 王潇，刘嘉睿，徐德均，朱斌健，徐俊延，敖国红，韩开宇，张长禹,2026,嗜卷书虱过氧化氢酶LbCAT基因的克隆及在高、低温胁迫下的表达分析[J].环境昆虫学报,(3):856-863 |
| 嗜卷书虱过氧化氢酶LbCAT基因的克隆及在高、低温胁迫下的表达分析 |
| Cloning of catalase gene LbCAT and its expression analysis under high and low temperature stresses in Liposcelis bostrychophila (Psopotera: Liposcelididae) |
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| DOI： |
| 中文关键词: 嗜卷书虱 过氧化氢酶 基因克隆 极端温度胁迫 表达分析 |
| 英文关键词:Liposcelis bostrychophila catalase gene cloning extreme temperature stress expression analysis |
| 基金项目:贵州中烟工业有限责任公司科技项目（GZZYKJBJ2020DWY0111，GZZYKJBJ2020DWY0121） |
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| 中文摘要: |
| 【目的】为探究嗜卷书虱Liposcelis bostrychophila过氧化氢酶CAT响应外界极端高、低温胁迫的分子机制。【方法】本研究克隆了嗜卷书虱CAT基因，命名为LbCAT，采用生物信息学方法和实时定量PCR（RT-qPCR）分析CAT基因的序列特征和在不同时长（0 min、60 min和120 min）、42℃高温和4℃低温胁迫下的相对表达量。【结果】结果表明，嗜卷书虱LbCAT基因（GenBank登录号：OQ938787）cDNA全长为1 693 bp，开放阅读框为1 404 bp，编码467个氨基酸，包括一个近端活性位点标签（26FDRERIPERVVHAKGAG42）和一个近端血红素配体标签（316RLFAYADTH324），具备CAT家族典型的结构域。系统发育分析显示，嗜卷书虱与同是啮虫目的嗜虫书虱的亲缘关系最近。高、低温胁迫均可诱导LbCAT表达，在4℃低温处理下，嗜卷书虱成虫LbCAT基因在60 min和120 min时的表达量与对照相比均极显著升高；在42℃高温处理下，嗜卷书虱成虫LbCAT基因在60 min和120 min时的表达量均极显著高于对照。【结论】研究结果表明LbCAT基因在嗜卷书虱响应极端温度胁迫的分子机制中可能发挥着重要作用。 |
| 英文摘要: |
| 【Aim】To investigate the molecular mechanism of catalase (CAT) responding to extreme high and low temperature stress in Liposcelis bostrychophila.【Methods】We cloned the CAT gene from this species and designated it LbCAT. Bioinformatics methods and real-time quantitative PCR (RT-qPCR) were employed to analyze the sequence features of LbCAT and its expression profiles under acute thermal stress (42℃ and 4℃) at 0, 60, and 120 min.【Results】The results showed that the full-length cDNA of the LbCAT gene (GenBank entry number: OQ938787) was 1 693 bp, and the open reading frame was 1 404 bp, encoding 467 amino acids. Sequence analysis revealed that LbCAT had a domain typical of the CAT family, including a proximal active site tag (26FDRERIPERVVHAKGAG42) and a proximal heme ligand tag (316RLFAYADTH324). Phylogenetic analysis showed that L. bostrychophila was closely related to Liposcelis entomophila, which also belongs to the order Psocoptera. The expression of the LbCAT gene was induced by both high and low temperature stress. At 4℃ temperature treatment, the expression of the LbCAT gene was significantly increased at 60 min and 120 min compared with the control. At 42℃ high-temperature treatment, the expression of the LbCAT gene at 60 min and 120 min was significantly higher than that of the control.【Conclusion】These results suggested that LbCAT may play a critical role in the molecular mechanism of L. bostrychophila responding to extreme thermal stress. |
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