| 霍昳陶，孙龙龙，刘永冲，殷新阔，苏广玲，刘龙，马英，汤清波,2026,GABAB受体介导糖代谢调控草地贪夜蛾幼虫对甜味物质的反应[J].环境昆虫学报,(3):666-676 |
| GABAB受体介导糖代谢调控草地贪夜蛾幼虫对甜味物质的反应 |
| GABAB receptor modulates responses to sweet substances by regulating sugar metabolism in Spodoptera frugiperda larvae |
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| DOI： |
| 中文关键词: 脂肪体代谢 GABAB受体 代谢信号 味觉感受 取食行为 |
| 英文关键词:Fat body metabolism GABAB receptor metabolic signals gustatory perception feeding behavior |
| 基金项目:河南省自然科学基金重点科学基金（232300421110）；河南省科技攻关项目（242102111091）；河南省太行山林业有害生物野外科学观测研究站开放课题（THFP202306） |
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| 中文摘要: |
| 【目的】前期研究发现代谢型受体γ氨基丁酸（γ-aminobutyric acid，GABA）B型受体（GABAB receptor，GABAB R）在草地贪夜蛾Spodoptera frugiperda幼虫脂肪体内高表达，并且显著影响幼虫对蔗糖的取食行为。然而，GABAB受体调控草地贪夜蛾幼虫取食的机制尚不清楚。【方法】本研究先后利用味觉单感器记录、转录组学、生理生化和行为检测技术研究GABAB受体介导的糖代谢对幼虫糖类取食行为的影响。【结果】GABAB R基因被干涉后，幼虫口器侧栓锥味觉感器对蔗糖的敏感性降低，体内糖代谢相关基因表达发生变化，体内葡萄糖和海藻糖浓度升高；分别向幼虫注射葡萄糖和海藻糖后，幼虫口器侧栓锥感器对蔗糖的敏感性均降低，且不再趋向取食蔗糖处理的叶碟。【结论】GABAB受体通过调控幼虫体内糖代谢影响糖类取食行为。本研究进一步揭示了神经递质GABA调控昆虫化学感受和取食行为的机制，为利用GABA防控草地贪夜蛾等农业害虫提供理论支持。 |
| 英文摘要: |
| 【Aim】Previous studies have found that the metabotropic γ-aminobutyric acid (GABA) B receptor (GABAB receptor, GABAB R) is highly expressed in the fat body of Spodoptera frugiperda larvae and significantly affects their feeding behavior in response to sucrose. However, the mechanism by which GABAB receptors regulate the feeding behavior of S. frugiperda larvae remains unclear.【Methods】In this study, single sensillum recording (SSR) of taste, transcriptomics, physiological and biochemical assays, and behavioral tests were used to investigate the effect of GABAB receptor-mediated sugar metabolism on larval sugar-feeding behavior.【Results】The results showed that after knockdown of the GABAB R gene, the sensitivity of the lateral styloconic sensilla on the larval mouthparts to sucrose decreased, the expression of sugar metabolism-related genes in the larvae changed, and the concentrations of glucose and trehalose in the larvae increased. Additionally, after separate injections of glucose or trehalose into larvae, the sensitivity of the lateral styloconic sensilla on the larval mouthparts to sucrose decreased, and the larvae no longer preferred to feed on sucrose-treated leaf discs.【Conclusion】These findings indicate that GABAB receptors affect larval sugar-feeding behavior by regulating sugar metabolism in larvae. This study further reveals the mechanism by which the neurotransmitter GABA regulates chemosensation and feeding behavior in insects, and provides theoretical support for the use of GABA in managing agricultural pests such as S. frugiperda. |
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