| 付冠军，周亚楠，唐睿，黄聪，钱万强，万方浩,2026,性别与交配状态对苹果蠹蛾感应寄主植物挥发物的影响[J].环境昆虫学报,(3):659-665 |
| 性别与交配状态对苹果蠹蛾感应寄主植物挥发物的影响 |
| Effects of sex and mating status on olfactory responses of Cydia pomonella to host plant volatiles |
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| DOI： |
| 中文关键词: 苹果蠹蛾 寄主植物挥发物 触角电位反应 交配状态 行为调控剂 |
| 英文关键词:Cydia pomonella host plant volatiles electroantennogram (EAG) mating status behavior-modifying agents |
| 基金项目:国家自然基金青年基金（32202303） |
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| 摘要点击次数: 217 |
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| 中文摘要: |
| 【目的】苹果蠹蛾Cydia pomonella是全球重要的入侵害虫，也是我国重点管控的检疫对象，主要危害苹果、梨等仁果类水果。近年来，随着苹果蠹蛾在我国入侵范围的扩大，非疫区防控压力日益严峻。本研究旨在筛选并验证寄主植物挥发物在苹果蠹蛾成虫嗅觉识别中的作用，为植物源引诱剂的开发提供理论依据。【方法】采用触角电位（Electroantennogram，EAG）技术，测定未交配和已交配苹果蠹蛾雌、雄成虫对27种植物源挥发物的EAG反应。【结果】在供试的27种化合物中，2,4-癸二烯酸乙酯（梨酯）、辛醛和壬醛3种化合物引起苹果蠹蛾成虫的EAG相对反应值显著高于对照（CK）。不同性别及交配状态的成虫对化合物的敏感性存在显著差异。浓度梯度实验表明，在一定范围内，EAG相对反应值随化合物浓度的增加呈剂量依赖性增强。梨酯在1 ng/μL浓度下即可引起雌虫显著的EAG反应，而雄虫在10 ng/μL时达到显著水平；辛醛和壬醛在1 μg/μL浓度下即可同时引起雌、雄成虫显著高于CK的EAG反应；此外，(E,E)-α-法尼烯在 10 μg/μL 浓度下表现出显著的EAG相对反应值。【结论】筛选的27种植物源挥发物均可在不同程度上引起苹果蠹蛾成虫的EAG反应，且成虫的嗅觉敏感性受性别、交配状态及化合物浓度的共同影响。其中，梨酯、辛醛、壬醛和(E,E)-α-法尼烯能够引起显著的成虫触角电位反应，是开发苹果蠹蛾植物源引诱剂或趋避剂的潜在候选化合物。 |
| 英文摘要: |
| 【Aim】The codling moth, Cydia pomonella, is a major global invasive pest and a priority quarantine pest in China, primarily damaging pome fruits such as apples and pears. In recent years, with the expanding invasion range of C. pomonella in China, the management pressure for prevention and control in non-infested areas has intensified. This study aims to screen and verify the role of host plant volatiles in the olfactory recognition of adult C. pomonella, providing a theoretical basis for the development of plant-derived attractants.【Methods】Electroantennography (EAG) was employed to measure the EAG responses of virgin and mated male and female adults to 27 host plant volatiles.【Results】Among the 27 tested compounds, ethyl 2,4-decadienoate (pear ester), octanal, and nonanal elicited relative EAG response values significantly higher than the control (CK). The sensitivity of adults to these compounds varied significantly according to sex and mating status. Concentration gradient experiments demonstrated that, within a certain range, relative EAG response values increased in a dose-dependent manner with increasing compound concentration. Specifically, pear ester elicited significant EAG responses in females at a concentration of 1 ng/μL, while males reached significant levels at 10 ng/μL. Octanal and nonanal elicited responses significantly higher than CK in both sexes at 1 μg/μL. Additionally, (E,E)-α-farnesene exhibited significant relative EAG response values at 10 μg/μL.【Conclusion】All 27 screened plant volatiles elicited EAG responses in C. pomonella adults to varying degrees. Olfactory sensitivity was jointly influenced by sex, mating status, and compound concentration. Notably, pear ester, octanal, nonanal, and (E,E)-α-farnesene elicited significant EAG responses in adult C. pomonella, indicating their potential as candidate compounds for the development of plant-derived attractants or repellents against this pest. |
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