| 彭震，李品，魏纪珍,2026,干扰钙调磷酸酶可导致棉铃虫死亡并引发显著的适合度代价[J].环境昆虫学报,(2):374-381 |
| 干扰钙调磷酸酶可导致棉铃虫死亡并引发显著的适合度代价 |
| Interfering with calcineurin induces mortality and imposes substantial fitness costs in Helicoverpa armigera |
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| DOI： |
| 中文关键词: 钙调磷酸酶 棉铃虫 RNAi 死亡率 生长发育 |
| 英文关键词:Calcineurin Helicoverpa armigera RNAi mortality growth and development |
| 基金项目:国家自然科学基金（32172401）；河南省高校科技创新人才项目（24HASTIT051）；中原青年拔尖人才项目 |
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| 中文摘要: |
| 【目的】钙调磷酸酶（Calcineurin，CaN）属于去磷酸化酶PP2B家族（Protein phosphatase 2B family），依赖于Ca2+信号的丝氨酸/苏氨酸蛋白磷酸酶，它在多种生物体的生理生化通路中具有广泛的调节作用。【方法】为探究CaN基因在棉铃虫Helicoverpa armigera中是否可作为RNA干扰（RNA interference, RNAi）农药研发的关键靶标基因，本研究对4龄幼虫进行CaN基因干扰，并系统分析了其致死率、体重变化、化蛹率、羽化率及其他相关生命参数的响应。【结果】结果表明，注射CaN dsRNA 可以有效的降低CaN基因的表达，并对应降低了CaN蛋白的酶活性。成功干扰CaN后，会导致棉铃虫4龄幼虫约60.81%的死亡，同时成活幼虫的体重自注射CaN dsRNA后24 h开始显著降低、从4龄幼虫至化蛹阶段以及蛹期至羽化阶段的发育时间均显著延长、化蛹率和羽化率亦显著下降，最终的羽化率仅为3.75%。【结论】综上所述，CaN是棉铃虫生长发育的重要基因，可以作为RNAi的一个重要靶标来进行棉铃虫的防治。 |
| 英文摘要: |
| 【Aim】Calcineurin (CaN), a Ca2?-dependent serine/threonine protein phosphatase belonging to the protein phosphatase 2B (PP2B) family, plays diverse regulatory roles in various physiological and biochemical pathways in vivo.【Methods】To investigate its potential as a key target gene for RNA interference (RNAi)-based pesticide development, this study conducted CaN RNAi on 4th instar Helicoverpa armigera and statistically analyzed their mortality, body weight, pupation rate, eclosion rate and other life indicators.【Results】The results showed that injection of CaN dsRNA significantly reduced both CaN gene expression and the enzymatic activity of CaN protein. Following knocking down CaN expression, about 60.81% mortality was observed among the fourth-instar larvae. Moreover, the body weight of surviving larvae showed a significant reduction starting 24 h after CaN dsRNA administration. The developmental durations from 4th instar larva to pupa and from pupa to adult eclosion were significantly prolonged. Both pupation and eclosion rates were markedly decreased, with the final eclosion rate dropping to only 3.75%.【Conclusion】These findings demonstrate that CaN is essential for the growth and development of H. armigera and highlight its potential as a promising RNAi target for pest control strategies. |
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