| 林天然，魏雅珍，杨晓敏，蒋桂花，蒋代兵，李亮生，彭凌飞,2026,RNAi在昆虫学研究中的递送方法及影响递送效率的因素[J].环境昆虫学报,(2):336-345 |
| RNAi在昆虫学研究中的递送方法及影响递送效率的因素 |
| Delivery methods of RNAi in entomological research and factors affecting delivery efficiency |
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| DOI： |
| 中文关键词: RNAi dsRNA 递送 研究方法 |
| 英文关键词:RNAi dsRNA delivery research methodology |
| 基金项目:中国烟草总公司福建省公司项目（2022350000240068）；福建省烟草公司龙岩市公司项目（2022350800240090） |
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| 中文摘要: |
| RNA干扰（RNAi）是一种由双链RNA（dsRNA）介导的基因沉默现象，该技术为生物学领域带来了重大变革，在基因功能研究、医疗、农业等各个领域均表现出巨大价值。RNAi对靶标基因的干扰效率受多重因素影响，其中dsRNA的递送方法对其影响很大。在昆虫学研究中，dsRNA的递送通常可通过注射、饲喂、体壁渗透（浸泡或喷洒）、转基因植物介导等方法进行，进而鉴别其基因功能或者进行其他应用开发。本文对这些递送方法进行了综述，并对影响dsRNA递送效率的一些关键因素进行了分析，以为众多的昆虫学研究以及该技术的应用方法提供参考。 |
| 英文摘要: |
| RNA interference (RNAi) is a gene silencing phenomenon caused by double-stranded RNA (dsRNA), which has brought about significant changes to the field of biology, and demonstrated great value in various areas such as gene function research, medicine, and agriculture. The efficiency of RNAi in interfering with target genes is influenced by multiple factors, among which the delivery method of dsRNA plays a crucial role. In entomological research, dsRNA can be delivered through methods such as injection, feeding, cuticle penetration (soaking or spraying), and transgenic plant-mediated delivery, enabling the identification of gene functions or other application developments. This article reviews these delivery methods and analyzes some key factors affecting dsRNA delivery efficiency, in order to provide references for numerous entomological studies and the application methodologies of this technology. |
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