| 王宸越，徐海珍，李沁颐，李 晶，张爱兵,,鳞翅目刺蛾科昆虫DNA条形码研究[J].环境昆虫学报,(): |
| 鳞翅目刺蛾科昆虫DNA条形码研究 |
| DNA barcoding of limacodidae (Lepidoptera) insects |
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| DOI： |
| 中文关键词: DNA条形码 刺蛾科 COI 序列 物种界定方法 ABGD方法 |
| 英文关键词:Limacodidae DNA barcoding COI sequence species delimitation ABGD |
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| 摘要点击次数: 279 |
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| 中文摘要: |
| 随着分子生物学和测序技术的快速发展，除BPSI、FuzzyID2、Niche barcoding、MMNet等物种识别方法外，DNA条形码（DNA barcoding）也已成为物种界定的重要工具。【目的】为评估不同物种界定方法在鳞翅目昆虫中的适用性。【方法】本研究以鳞翅目刺蛾科昆虫（Lepidoptera：Limacodidae）为研究对象，整合NCBI、BOLD等公共数据库及自主采集的4 563条COI（Cytochrome C Oxidase subunit I）序列数据（涵盖176个形态学物种），基于jMOTU、ABGD和bPTP物种界定方法系统比较分析了多种条形码界定方法在刺蛾科昆虫中的分类效能。【结果】结果表明，COI条码在刺蛾科种级水平分类中适用性很高，64%的形态学物种在系统发育树中形成高支持率单系群，仅在部分近缘属间存在一定的非单系现象。在本研究测试的物种界定方法中，ABGD方法综合表现最优（平均准确率达93% ± 1.8%），其基于遗传距离自动检测物种边界的算法显著提升了物种界定的稳健性；而bPTP模型因过度划分导致准确率最低（60.01%）。同时，研究发现刺蛾科种内与种间遗传距离存在重叠现象，这可能与隐存种复合体等相关。【结论】本研究通过比较多种物种界定方法，进一步证明DNA条形码在鳞翅目刺蛾科昆虫分类中的适用性，可以为DNA条形码技术的广泛应用提供参考。 |
| 英文摘要: |
| With advances in molecular techniques, in addition to species identification methods such as BPSI, FuzzyID2, Niche barcoding, and MMNet, DNA barcoding has become a pivotal tool for species delimitation. 【Aim】This study systematically evaluated species delineation approaches within Lepidoptera, with particular emphasis on Limacodidae moths. 【Methods】By integrating 4 563 COI sequences from NCBI, BOLD, and original field collections (representing 176 morphospecies), we employed multiple analytical frameworks —jMOTU, ABGD, and the bPTP model— to assess genetic characteristics and taxonomic resolution. 【Results】Our findings demonstrate that COI markers effectively resolved species-level delimitation, with 64% of morphospecies forming strongly supported monophyletic clades. However, we observed phylogenetic ambiguities among congeneric lineages. Methodological comparisons revealed that the ABGD approach achieved superior accuracy (93% ± 1.8%), contrasting with the limited performance of the bPTP model (60.01% accuracy). Notable overlap between interspecific and intraspecific genetic distances suggests cryptic speciation or other driving forces. 【Conclusion】While confirming the operational utility of DNA barcoding for Limacodidae systematics, this comparative study further substantiates the applicability of the DNA barcoding in species delimitation for this group. Our findings provide valuable reference for the broader application of DNA barcoding technology in taxonomic research. |
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