| 姚佳运，李治模，董祥立，张长禹，杨昌利，孟建玉,,蠋蝽对高温胁迫响应的转录组分析[J].环境昆虫学报,(): |
| 蠋蝽对高温胁迫响应的转录组分析 |
| Transcriptome analysis of the response of Arma chinensis to high temperature stress |
| |
| DOI： |
| 中文关键词: 蠋蝽 转录组分析 温度胁迫 热激蛋白 |
| 英文关键词:Arma chinensis transcriptome analysis temperature stress heat shock protein |
| 基金项目: |
|
| 摘要点击次数: 468 |
| 全文下载次数: 188 |
| 中文摘要: |
| 蠋蝽Arma chinensis是一类杂食性天敌昆虫，其捕食能力强、捕食范围广，在生物防治中有广泛应用前景。为探究蠋蝽对极端高温的适应机制，本研究采用Illumina高通量测序技术，对高温38℃处理0 h（Control）、6 h（HT6h）、12 h（HT12h）后的蠋蝽成虫进行转录组分析。结果表明，HT6h vs Control共有1 613个差异表达基因，其中768个上调和845个下调；HT12h vs Control共有2 023个差异表达基因，其中789个上调和1 234个下调；HT12h vs HT6h共有699个差异表达基因，其中320个上调和379个下调。KEGG富集分析结果表明，差异表达基因主要参与氧化磷酸化、药物代谢酶细胞色素P450等通路。与温度相关的基因HSP（Heat shock protein）、DnaJ（DnaJ homolog subfamily）、CYP450（Cytochrome P450）、UGT（UDP-glucuronosyltransferase）等表现为上调，表明这些蛋白基因可能参与蠋蝽对极端温度的耐受性作用。采用实时荧光定量PCR（qRT-PCR）对随机筛选的10个基因进行验证，测定结果与转录组测定结果一致，表明转录组测序数据准确可信。该研究揭示了蠋蝽温度胁迫响应相关基因的表达特征，为探索蠋蝽适应高温胁迫的分子机理奠定了基础。 |
| 英文摘要: |
| The predatory stinkbug Arma chinensis is an omnivorous natural enemy insect with strong predation ability and a broad prey range, showing great potential for widespread application in biological control. To explore the adaptation mechanism of A. chinensis to extreme high temperatures, this study performed transcriptome analysis on adult A. chinensis after treatment at 38℃ for 0 h (Control), 6 h (HT6h) and 12 h (HT12h) using Illumina high-throughput sequencing technology. The results showed that there were 1 613 differentially expressed genes in HT6h vs Control, among which 768 were up-regulated and 845 were down-regulated. In HT12h vs Control, there were 2 023 differentially expressed genes , with 789 up-regulated and 1 234 down-regulated. For HT12h vs HT6h, a total of 699 differentially expressed genes were identified, including 320 up-regulated and 379 down-regulated ones. KEGG pathway enrichment analysis indicated that these differentially expressed genes were mainly involved in oxidative phosphorylation, drug metabolism-cytochrome P450, and other related pathways. Notably, temperature-related genes such as HSP (Heat shock protein), DnaJ (DnaJ homolog subfamily), CYP450 (Cytochrome P450) and UGT (UDP-glucuronosyltransferase) were up-regulated, indicating that these protein genes might be involved in the tolerance of A. chinensis to extreme temperatures. 10 randomly selected genes were verified using real-time fluorescence quantitative PCR (qRT-PCR), and the results were consistent with those of the transcriptome analysis, indicating that the transcriptome sequencing data is accurate and reliable. This study reveals the expression characteristics of genes related to the response of A. chinensis to temperature stress, laying a foundation for exploring the molecular mechanism underlying the adaptation of A. chinensis to high temperature stress. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
