| 孙函函，廖江花，李 超,2025,转ACT基因马铃薯对马铃薯甲虫生长发育和酶活性的影响[J].环境昆虫学报,(6):1888-1898 |
| 转ACT基因马铃薯对马铃薯甲虫生长发育和酶活性的影响 |
| Effect of transgenic ACT potato on growth, development and enzyme activity of Leptinotarsa decemlineata |
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| DOI： |
| 中文关键词: 马铃薯甲虫 转ACT基因马铃薯 生长发育 解毒酶 保护酶 |
| 英文关键词:Leptinotarsa decemlineata transgenic ACT potatoes growth and development detoxification enzyme protective enzyme |
| 基金项目:国家重点研发计划（2021YFD1400200）；国家自然科学基金（31660545）；农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室开放基金（KFJJ201905）；新疆维吾尔自治区天山英才计划资助 |
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| 中文摘要: |
| 马铃薯甲虫Leptinotarsa decemlineata作为世界性检疫害虫之一，对茄科作物造成的危害是毁灭性的。通过对马铃薯植株靶向β-Actin（ACT）转基因可以诱导马铃薯甲虫的RNAi反应致死。本文探究了转ACT基因、野生型WT和经济品种荷兰15马铃薯对马铃薯甲虫发育存活和酶活性的影响。结果表明，各龄期马铃薯甲虫取食转ACT基因叶片后，其消耗叶片面积会随取食天数的增加而减少、虫体重量随天数增加而减轻（P < 0.05）。马铃薯甲虫2龄、3龄、4龄幼虫和成虫取食转ACT基因叶片后的解毒酶（CarE，GST，AKP/ALP）、乙酰胆碱酯酶（AChE）和保护酶（SOD，CAT，POD）酶活性显著高于取食野生型WT和荷兰15（P < 0.05）。各龄期马铃薯甲虫取食转ACT基因、野生型WT和荷兰15块茎后生长发育和酶活性没有显著性差异。本研究通过探究转ACT马铃薯对马铃薯甲虫生长发育及酶活性的影响，为转ACT基因马铃薯的安全性以及可持续的防治方法对抗害虫提供依据。 |
| 英文摘要: |
| The Colorado potato beetle, Leptinotarsa decemlineata, as one of the world quarantine pests, causes devastating the damage to solanaceae crops. Targeting the β-Actin (ACT) transgene in potato plants can induce a lethal RNAi response of CPBs. In this study, we investigated the effects of different potato varieties (transgenic ACT, wild-type WT and commercial variety Holland 15) on the development, survival and enzyme activity of CPB. The results showed that after CPBs at various developmental stages feeding on ACT leaves, both the leaf area consumed and the body weight decreased with increasing feeding duration (P < 0.05). The activities of detoxification enzymes (CarE, GST, AKP/ALP), acetylcholinesterase (AChE), and protective enzymes (SOD, CAT, POD) in the 2nd, 3rd and 4th instar larvae and adults of CPB were significantly higher in beetles fed on transgenic ACT than those of wild-type WT and Holland 15 (P < 0.05). However, there was no significant difference in the growth, development, or enzyme activity of CPBs after feeding on transgenic ACT, wild-type WT and Holland 15 potato tubers at different instar stages. This paper explores the effects of transgenic ACT potatoes on the development, survival and enzyme activity of CPBs. It provides insights into the safety of transgenic ACT potatoes and supports sustainable control methods against pests. |
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