| 马 骏，刘 盼，曹文泽，李凯兵，陈 萍，胡淑青，徐 强，王兴民，武目涛，刘海军,2025,长林小蠹基于重测序SNP标记的种群溯源技术[J].环境昆虫学报,(6):1725-1734 |
| 长林小蠹基于重测序SNP标记的种群溯源技术 |
| Population traceability technology of Hylurgus ligniperda Fabricius (Coleoptera: Curculionidae) based on resequencing-derived SNP markers |
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| DOI： |
| 中文关键词: 长林小蠹 基因组重测序 SNP标记 种群溯源 种群结构 |
| 英文关键词:Hylurgus ligniperda genomic resequencing SNP population traceability population structure |
| 基金项目:国家重点研发计划（2021YFD1400104） |
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| 中文摘要: |
| 长林小蠹Hylurgus ligniperda是一种对松树具有严重危害的林业入侵性害虫，在中国属于进境植物检疫性有害生物。本研究针对来自澳大利亚、新西兰、俄罗斯和中国山东的长林小蠹地理种群做了基因组重测序，基于SNP位点的种群遗传分化分析结果表明，中国、新西兰、俄罗斯和澳大利亚的长林小蠹组成相互独立种群结构，其中中国种群与新西兰种群的遗传距离较近，而与俄罗斯、澳大利亚种群的遗传距离较远。通过种群特异性SNP溯源位点筛查，成功建立了中国、新西兰和俄罗斯种群的SNP溯源位点，能够准确识别目标种群的基因型，为口岸检疫和疫区防控提供了快速、可靠的分子溯源技术。研究结果表明，我国分布的长林小蠹种群可能直接或间接来源于新西兰，为口岸长林小蠹检疫监管提供了参考。 |
| 英文摘要: |
| Hylurgus ligniperda (Coleoptera: Curculionidae), a highly destructive forestry invasive pest to pine trees, is a quarantine-regulated species for entry plants in China. We conducted genomic resequencing on geographical populations of H. ligniperda collected from Australia, New Zealand, Russia, and Shandong Province, China. Population genetic differentiation analysis based on Single Nucleotide Polymorphism (SNP) loci revealed distinct genetic clusters corresponding to the Chinese, New Zealand, Russian, and Australian populations. Specifically, the Chinese population exhibited closer genetic proximity to the New Zealand population but greater divergence from the Russian and Australian populations. We screened and successfully established population-specific SNP traceability loci for the Chinese, New Zealand, and Russian populations. All markers were validated for accurately identifying target population genotypes, providing rapid and reliable molecular tracing technology for port inspections and quarantine area management. Additionally, our results indicate that the Chinese population of H. ligniperda may have originated directly or indirectly from New Zealand, offering critical clues for quarantine regulation of this pest at Chinese ports. |
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