| 王柄权，程凌寒，方宇，刘玲玲，余磊，羊绍武，齐颖，刘佳妮,2025,西花蓟马对番茄防御响应的转录组学分析[J].环境昆虫学报,(5):1626-1636 |
| 西花蓟马对番茄防御响应的转录组学分析 |
| Transcriptome analysis of Frankliniella occidentalis response to tomato defense mechanisms |
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| DOI： |
| 中文关键词: 西花蓟马 番茄 植物防御 转录组 |
| 英文关键词:Frankliniella occidentalis tomato plant defense transcriptome |
| 基金项目:云南省科技厅联合专项重点项目（2019FH001-008） |
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| 摘要点击次数: 432 |
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| 中文摘要: |
| 为揭示西花蓟马Frankliniella occidentalis对番茄寄主的适应机制，探究西花蓟马响应番茄防御反应的转录组差异，本研究采用Illumina测序技术对取食番茄2 h（瞬时响应）和取食番茄24 h（长时间响应）下西花蓟马转录组进行分析，并结合GO和KEGG富集，比较分析筛选出西花蓟马适应番茄寄主的相关基因。结果表明：与对照处理相比，瞬时响应和长时间响应处理中分别有72.79%和71.92%的差异表达基因上调表达，且随着取食时间的延长差异表达基因的上调数量逐渐增多；GO富集分析显示西花蓟马在瞬时和长时间响应下，生物过程的相关代谢和感知觉规律，分子功能的转运活性、角质层的结构组成和分子结构活性等相关基因可能在西花蓟马适应番茄的过程中发挥着重要作用。KEGG富集分析显示西花蓟马在适应番茄寄主防御调控机制中主要与能量的代谢与分配、调控信号转导和激活免疫应激等通路密切相关。此外，对不同比较组中与消化酶和解毒酶相关的差异基因进行分析，结果显示瞬时响应vs对照处理中消化酶（23个胰蛋白酶和6个脂肪酶）、解毒酶（14个细胞色素P450和12个羧酸酯酶家族）；长时间响应vs对照处理中消化酶（33个胰蛋白酶和13个脂肪酶）、解毒酶（28个细胞色素P450和16个羧酸酯酶家族）；长时间响应vs瞬时响应中消化酶（12个胰蛋白酶和3个脂肪酶）、解毒酶（10个细胞色素P450和4个羧酸酯酶家族），且上述消化酶和解毒酶整体趋势表现为上调。总而言之，西花蓟马在响应番茄防御过程中与能量代谢、信号转导以及免疫等相关的基因较为活跃，且与消化酶和解毒酶相关的差异表达基因主要以上调表达的形式参与西花蓟马适应番茄寄主的过程。 |
| 英文摘要: |
| To reveal the adaptation mechanism of Frankliniella occidentalis to tomato hosts and investigate its transcriptomic differences responding to plant defense, we used illumina sequencing to analyze the transcriptomes of F. occidentalis fed on tomato plants for 2 h (instantaneous response) and 24 h (long-term response); combined with GO and KEGG enrichment analyses, comparative transcriptomics identified key genes involved in the thrips' adaptation to host defense. The results revealed that compared with the control group, 72.79% and 71.92% of differentially expressed genes (DEGs) were up-regulated after 2 h and 24 h feeding treatments, respectively, with the number of up-regulated DEGs increasing over time. GO enrichment analysis indicated that under both instantaneous and long-term responses, genes associated with biological processes (including metabolic processes and sensory perception), as well as molecular functions (such as transporter activity, structural constituent of cuticle and molecular structural activity) potentially mediate the adaptation of F. occidentalis to tomato defense. KEGG enrichment analysis highlighted significant involvement of pathways associated with energy metabolism and allocation, signal transduction, and immune responses in mediating F. occidentalis adaptation to host defense regulation. Furthermore, we analyzed DEGs associated with digestive and detoxification enzymes across comparison groups. The results revealed that in the 2 h feeding group versus control, digestive enzymes (23 trypsins and 6 lipases), detoxification enzymes (14 cytochrome P450 and 12 carboxylesterase families) were detected; in the 24 h feeding group versus control, digestive enzymes (33 trypsins and 13 lipases), detoxification enzymes (28 cytochrome P450 and 16 carboxylesterase families) were observed; and in the 24 h versus 2 h comparison, digestive enzymes (12 trypsins and 3 lipases), detoxification enzymes (10 cytochrome P450 and 4 carboxylesterase families) were observed. Notably, the overall trend for these digestive and detoxification enzymes was up-regulated In summary, Genes associated with energy metabolism, signal transduction, and immunity exhibited heightened activity in F. occidentalis?responding to tomato defense mechanisms, while DEGs encoding digestive and detoxification enzymes primarily mediated thrips adaptation to tomato plants through up-regulated expression. |
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