| 郑美鑫，张茜瑜，李蕙，宋潇洋，郭嘉庆，张雪，张俊杰,2025,松毛虫赤眼蜂两种海藻糖转运蛋白基因的克隆与表达分析[J].环境昆虫学报,(5):1570-1578 |
| 松毛虫赤眼蜂两种海藻糖转运蛋白基因的克隆与表达分析 |
| Cloning and expression analysis of two trehalose transporter genes in Trichogramma dendrolimi |
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| DOI： |
| 中文关键词: 松毛虫赤眼蜂 海藻糖转运蛋白基因 基因克隆 表达模式 |
| 英文关键词:Trichogramma dendrolimi trehalose transporter gene cloning expression pattern |
| 基金项目:国家青年科学基金（32102291）；长春市科技发展计划（21ZGN14） |
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| 中文摘要: |
| 本研究旨在分析松毛虫赤眼蜂Trichogramma dendrolimi两种海藻糖转运蛋白基因（TdTret1和TdTret1-2）的表达模式，和其在松毛虫赤眼蜂不同发育阶段及滞育发育过程中的基因表达规律，为海藻糖调节昆虫抵御低温环境及松毛虫赤眼蜂滞育的调控机制的研究提供理论基础。对两种基因进行克隆和定量试验，结果表明，TdTret1和TdTret1-2均隶属于MFS超家族，与膜翅目多种昆虫的海藻糖转运蛋白氨基酸序列进行比较分析，TdTret1和TdTret1-2均与短管赤眼蜂序列一致性最高，亲缘关系最近；这两种海藻糖转运蛋白基因在松毛虫赤眼蜂内体呈现“错峰”表达的模式，其中TdTret1主要在松毛虫赤眼蜂生长发育的早期阶段（卵期、幼虫期）高表达，而TdTret1-2则是在松毛虫赤眼蜂的卵期、蛹期和成虫期特异性高表达；TdTret1和TdTret1-2在滞育松毛虫赤眼蜂个体中的表达量显著高于非滞育个体，但两种基因高表达的时间点不同，TdTret1-2相较于TdTret1对低温的响应更迅速。这两种海藻糖转运蛋白基因在松毛虫赤眼蜂正常发育及滞育发育过程中均发挥重要作用。 |
| 英文摘要: |
| The purpose of this study was to analyze the expression patterns of two trehalose transporter genes (TdTret1 and TdTret1-2) and gene expression patterns at different developmental stages and diapause development in Trichogramma dendrolimi. This analysis aimed to provide a theoretical basis for understanding the regulation of trehalose in insects to adapt to low temperature environments and the regulatory mechanism of diapause in T. dendrolimi. Gene cloning and quantitative analysis of the two types of trehalose transporter genes indicated that TdTret1 and TdTret1-2 belong to the MFS superfamily. Amino acid sequences of trehalose transporters were compared with those of hymenoptera insects, revealing that TdTret1 and TdTret1-2 had the highest consistency with Trichogramma pretiosum, suggesting a close genetic relationship. These two trehalose transporter genes exhibited a "staggered" expression pattern in T. dendrolimi. TdTret1 was mainly expressed in the early growth stages (i.e., egg stage and larval stage), while TdTret1-2 showed significantly higher expression levels during the egg, pupal and adult stages. Both TdTret1 and TdTret1-2 were significantly upregulated in diapausing T. dendrolimi compared to non-diapausing ones, but with distinct timing; it was observed that TdTret1-2 responded more quickly to low temperatures than TdTret1. These two trehalose transporter genes play an important role in the normal development and diapause development of T. dendrolimi. |
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