| 王莉娇，饶中臣，廖慧琳，韩日畴，金永玲，曹莉,2025,硫酸卡那霉素和盐酸萘替芬促进冬虫夏草菌二型态转化的相关基因和通路分析[J].环境昆虫学报,(5):1548-1569 |
| 硫酸卡那霉素和盐酸萘替芬促进冬虫夏草菌二型态转化的相关基因和通路分析 |
| Analysis of the related genes and pathways related to dimorphic transition of Ophiocordyceps sinensis promoted by kanamycin sulfate and naftifine hydrochloride |
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| DOI： |
| 中文关键词: 冬虫夏草菌 二型态 硫酸卡那霉素 盐酸萘替芬 转录组 |
| 英文关键词:Ophiocordyceps sinensis dichotypy kanamycin sulfate naftifine hydrochloride transcriptome |
| 基金项目:广东省科学院发展专项资金项目（2024GDASZH-2024010101）；广东省重点领域研发计划项目（2020B1111580001） |
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| 中文摘要: |
| 冬虫夏草（Chinese cordyceps）是冬虫夏草菌Ophiocordyceps sinensis寄生于鳞翅目蝙蝠蛾科Hepialidae昆虫幼虫上形成的虫菌复合体，具有抗疲劳、抗衰老、治疗肺肾疾病等多种功效。虽然冬虫夏草人工培育技术业已建立，但冬虫夏草菌进入寄主后从芽生孢子转化为菌丝（即二型态转化）再形成僵虫的效率直接影响人工培育的成本。为促进冬虫夏草菌二型态转化，本研究分析了冬虫夏草菌响应外源化合物硫酸卡那霉素（Kanamycin sulfate）或盐酸萘替芬（Naftifine hydrochloride）的转录组，共获得2 801个差异表达基因。富集分析结果显示，硫酸卡那霉素处理芽生孢子3 d的上下调的基因主要富集于碳水化合物代谢途径，而盐酸萘替芬处理芽生孢子3 d的上调基因主要富集于谷胱甘肽代谢、脂肪酸分解和过氧化物酶体等通路；下调基因均显著富集于糖酵解、TCA循环、脂肪酸合成、核糖体和转录翻译等相关通路。研究结果为阐明冬虫夏草菌二型态转化的分子机制及提升冬虫夏草人工培育效率提供新思路。 |
| 英文摘要: |
| Chinese cordyceps, a parasitic complex formed by the larvae of Hepialidae (Lepidoptera) and the fungus Ophiocordyceps sinensis, exhibits various therapeutic properties, such as anti-fatigue, anti-aging effects, and the treatment of pulmonary and renal diseases. Although artificial cultivation techniques for Chinese cordyceps have been established, the dimorphic transition of O. sinensis, from blastospores to hyphae after infecting the host, and ultimately forming mummified insect, remains a major bottleneck that directly affects the cultivation efficiency and cost. To promote this dimorphic transition, transcriptome analysis was performed on blastospores treated with exogenous compounds—kanamycin sulfate and naftifine hydrochloride, resulting in the identification of 2 801 differentially expressed genes (DEGs). Enrichment analysis revealed that, after three days of kanamycin sulfate treatment, both up- and down-regulated genes were predominantly associated with carbohydrate metabolism. In contrast, in naftifine hydrochloride-treated spores over three days, up-regulated genes were mainly enriched in glutathione metabolism, fatty acid degradation, and peroxisome pathways, while down-regulated genes were significantly enriched in glycolysis, the tricarboxylic acid (TCA) cycle, fatty acid biosynthesis, ribosome, and transcription/translation processes. These findings provide novel insights into the molecular mechanisms of the dimorphic transition in O. sinensis and suggest potential strategies for improving the efficiency of its artificial cultivation. |
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