| 成印洁，常海龙，刘键柏，毛永凯，李继虎，张建华,2025,玉米MicroRNAs响应草地贪夜蛾取食胁迫的功能分析[J].环境昆虫学报,(5):1457-1466 |
| 玉米MicroRNAs响应草地贪夜蛾取食胁迫的功能分析 |
| Functional analysis of microRNAs in maize responding to feeding stress of the fall armyworm (Spodoptera frugiperda) |
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| DOI： |
| 中文关键词: 草地贪夜蛾 玉米 miRNA 肌醇磷酸代谢通路 基因剪切信号通路 |
| 英文关键词:Spodoptera frugiperda Zea mays miRNA inositol phosphate metabolic pathway gene splicing signaling pathway |
| 基金项目:广东省科学院科技发展项目（2020GDASYL-20200103068）；广州市重点研发计划（2024B03J1272） |
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| 中文摘要: |
| 草地贪夜蛾Spodoptera frugiperda是玉米上的一种重大迁飞性害虫，严重危害玉米产量和粮食安全。为阐明玉米响应草地贪夜蛾取食胁迫防御反应的分子机制，利用高通量测序技术对草地贪夜蛾3龄幼虫取食的玉米叶片进行微小RNA（MicroRNAs，miRNA）组学测序并结合生物信息学分析方法对miRNA的靶基因进行了预测和分析。结果显示，在草地贪夜蛾取食1 d和3 d的玉米叶片内，共检测到500个miRNAs。草地贪夜蛾取食诱导1 d后检测到317个miRNAs差异表达，其中93个miRNAs是玉米叶片被草地贪夜蛾取食1 d后特异检测到的；草地贪夜蛾取食诱导3 d后检测到309个miRNAs差异表达，其中90个miRNAs是玉米叶片被草地贪夜蛾取食3 d后特异检测到的。这些差异表达的miRNAs共预测到170 003个靶基因，靶基因主要富集在蛋白质结合、DNA结合和细胞核等区域，这些基因主要富集在肌醇磷酸代谢以及基因剪切相关信号通路上。通过RT-qPCR验证了草地贪夜蛾取食1 d和3 d的玉米叶片上调表达的部分miRNA，与生物信息学预测的结果一致。本研究增加了对草地贪夜蛾取食诱导玉米中miRNAs的差异表达及其调控的信号通路的了解，并进一步揭示了介导昆虫—宿主植物相互作用的复杂调控机制。本研究结果将为miRNAs在调控昆虫与寄主植物相互作用中的功能提供一定的理论基础，并为作物抗虫提供新的调控靶点。 |
| 英文摘要: |
| The fall armyworm, Spodoptera frugiperda, is a major migratory pest of maize, severely endangering maize yield and food security. To elucidate the molecular mechanism of defense responses of maize to S. frugiperda feeding stress, microRNA (miRNA) transcriptome sequencing was performed using high-throughput sequencing technology on maize leaves consumed by the 3rd-instar larvae of S. frugiperda, combined with bioinformatics analysis methods for prediction and analysis of miRNAs target genes. The results showed that a total of 500 miRNAs were detected in maize leaves after one day (d) and 3 d of S. frugiperda feeding. After 1 d of feeding induction, 317 miRNAs were differentially expressed, of which 93 miRNAs were specifically detected in maize leaves post 1 d of S. frugiperda feeding. After 3 d of feeding induction, 309 miRNAs were differentially expressed, with 90 miRNAs specifically detected in maize leaves post 3 d of S. frugiperda feeding. A total of 170,003 target genes were predicted for these differentially expressed miRNAs. These target genes were primarily enriched in categories such as protein binding, DNA binding, and cell nucleus. Furthermore, these genes were mainly enriched in signaling pathways related to inositol phosphate metabolism and gene splicing. The upregulated expression of selected miRNAs in maize leaves at 1 d and 3 d post-S. frugiperda feeding was validated by RT-qPCR, which was consistent with the bioinformatics predictions. This study enhances our understanding of the differential expression of miRNAs and their regulated signaling pathways in maize induced by S. frugiperda feeding, and further reveals the complex regulatory mechanisms mediating insects and host plants interaction. The findings will provide a theoretical basis for the function of miRNAs in regulating insects and host plants interaction and offer new regulatory targets for crop insect resistance. |
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